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首页> 外文期刊>Journal of Molecular Biology >DETERMINATION OF DNA PERSISTENCE LENGTH BY CRYO-ELECTRON MICROSCOPY - SEPARATION OF THE STATIC AND DYNAMIC CONTRIBUTIONS TO THE APPARENT PERSISTENCE LENGTH OF DNA
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DETERMINATION OF DNA PERSISTENCE LENGTH BY CRYO-ELECTRON MICROSCOPY - SEPARATION OF THE STATIC AND DYNAMIC CONTRIBUTIONS TO THE APPARENT PERSISTENCE LENGTH OF DNA

机译：电子显微镜测定DNA的持久力-分离DNA的静态持久力的静态和动态贡献。

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摘要

Axial deflection of DNA molecules in solution results from thermal motion and intrinsic curvature related to the DNA sequence. In order to measure directly the contribution of thermal motion we constructed intrinsically straight DNA molecules and measured their persistence length by cryo-electron microscopy The persistence length of such intrinsically straight DNA molecules suspended in thin layers of cryo-vitrified solutions is about 80 nm. In order to test our experimental approach, we measured the apparent persistence length of DNA molecules with natural ''random'' sequences. The result of about 45 nm is consistent with the generally accepted value of the apparent persistence length of natural DNA sequences. By comparing the apparent persistence length to intrinsically straight DNA with that of natural DNA, it is possible to determine both the dynamic and the static contributions to the apparent persistence length. (C) 1995 Academic Press Limited [References: 45]
机译：DNA分子在溶液中的轴向偏转是由与DNA序列相关的热运动和固有曲率引起的。为了直接测量热运动的贡献，我们构建了固有的直链DNA分子，并通过冷冻电子显微镜测量了其持久性长度。这种固有的直链DNA分子悬浮在低温玻璃化溶液的薄层中的持久性长度约为80 nm。为了测试我们的实验方法，我们测量了具有自然“随机”序列的DNA分子的表观持久性长度。约45nm的结果与天然DNA序列的表观持久性长度的普遍接受的值一致。通过比较固有的直链DNA的表观持久性长度和天然DNA的持久性长度，可以确定对表观持久性长度的动态和静态贡献。 （C）1995 Academic Press Limited [参考号：45]

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