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首页> 外文期刊>Journal of neuroendocrinology >Neuronal Gonadotrophin-Releasing Hormone (GnRH) and Astrocytic Gonadotrophin Inhibitory Hormone (GnIH) Immunoreactivity in the Adult Rat Hippocampus
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Neuronal Gonadotrophin-Releasing Hormone (GnRH) and Astrocytic Gonadotrophin Inhibitory Hormone (GnIH) Immunoreactivity in the Adult Rat Hippocampus

机译：成年大鼠海马中的神经元促性腺激素释放激素（GnRH）和星形胶质促性腺激素抑制激素（GnIH）免疫反应性

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摘要

Gonadotrophin-releasing hormone (GnRH) and gonadotrophin inhibitory hormone (GnIH) are neuropeptides secreted by the hypothalamus that regulate reproduction. GnRH receptors are not only present in the anterior pituitary, but also are abundantly expressed in the hippocampus of rats, suggesting that GnRH regulates hippocampal function. GnIH inhibits pituitary gonadotrophin secretion and is also expressed in the hippocampus of a songbird; its role outside of the reproductive axis is not well established. In the present study, we employed immunohistochemistry to examine three forms of GnRH [mammalian GnRH-I (mGnRH-I), chicken GnRH-II (cGnRH-II) and lamprey GnRH-III (lGnRH-III)] and GnIH in the adult rat hippocampus. No mGnRH-I and cGnRH-II+ cell bodies were present in the hippocampus. Sparse mGnRH-I and cGnRH-II+ fibres were present within the CA1 and CA3 fields of the hippocampus, along the hippocampal fissure, and within the hilus of the dentate gyrus. No lGnRH-III was present in the rodent hippocampus. GnIH-immunoreactivity was present in the hippocampus in cell bodies that resembled astrocytes. Males had more GnIH+ cells in the hilus of the dentate gyrus than females. To confirm the GnIH+ cell body phenotype, we performed double-label immunofluorescence against GnIH, glial fibrillary acidic protein (GFAP) and NeuN. Immunofluorescence revealed that all GnIH+ cell bodies in the hippocampus also contained GFAP, a marker of astrocytes. Taken together, these data suggest that GnRH does not reach GnRH receptors in the rat hippocampus primarily via synaptic release. By contrast, GnIH might be synthesised locally in the rat hippocampus by astrocytes. These data shed light on the sites of action and possible functions of GnRH and GnIH outside of the hypothalamic-pituitary-gonadal axis.
机译：促性腺激素释放激素（GnRH）和促性腺激素抑制激素（GnIH）是下丘脑分泌的调节再生的神经肽。 GnRH受体不仅存在于垂体前叶，而且在大鼠海马中大量表达，提示GnRH调节海马功能。 GnIH抑制垂体促性腺激素的分泌，并在鸣禽的海马中表达。它在生殖轴外的作用尚不明确。在本研究中，我们采用免疫组化技术检查了成人中GnRH的三种形式[哺乳动物GnRH-I（mGnRH-I），鸡GnRH-II（cGnRH-II）和七lamp鳗GnRH-III（lGnRH-III）]和GnIH大鼠海马。海马中不存在mGnRH-I和cGnRH-II +细胞体。稀疏的mGnRH-I和cGnRH-II +纤维存在于海马的CA1和CA3区域内，沿海马裂隙以及齿状回的希拉内。啮齿动物海马中不存在lGnRH-III。 GnIH免疫反应性存在于海马中类似于星形胶质细胞的细胞体中。雄性在齿状回的hilus中比雌性拥有更多的GnIH +细胞。为了确认GnIH +细胞体表型，我们对GnIH，神经胶质原纤维酸性蛋白（GFAP）和NeuN进行了双标记免疫荧光。免疫荧光显示，海马中的所有GnIH +细胞体也都含有星形胶质细胞标记物GFAP。综上所述，这些数据表明，GnRH主要不是通过突触释放到达大鼠海马中的GnRH受体。相比之下，GnIH可能是星形胶质细胞在大鼠海马中局部合成的。这些数据揭示了下丘脑-垂体-性腺轴外GnRH和GnIH的作用部位和可能的功能。

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