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首页> 外文期刊>Journal of Neuroimmunology: Official Bulletin of the Research Committee on Neuroimmunology of the World Federation of Neurology >T-cell response to myelin basic protein and lipid-bound myelin basic protein in patients with multiple sclerosis and healthy donors.
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T-cell response to myelin basic protein and lipid-bound myelin basic protein in patients with multiple sclerosis and healthy donors.

机译：多发性硬化患者和健康捐献者对髓鞘碱性蛋白和脂质结合的髓鞘碱性蛋白的T细胞反应。

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摘要

An autoimmune T-cell response to myelin proteins is thought to be involved in the pathogenesis of multiple sclerosis (MS) and myelin basic protein (MBP) is the most widely studied potential target antigen. We investigated the T-cell response to MBP in MS patients and controls using two different molecular forms of the protein: the classical hydrophilic MBP (lipid-free MBP, LF-MBP) and a lipid-bound, native-like preparation of MBP isolated in a molecular form retaining the binding to all myelin lipids (lipid-bound-MBP, LB-MBP). Short term T-cell lines (TCL) were generated using either LF- or LB-MBP and tested for their reactivity to the in vitro stimulating antigen. No differences were detected between MS patients and healthy donors in the percentage of T-cell cultures responsive to the LF-MBP. In contrast, the number of LB-MBP reactive cultures was higher in MS patients than in controls. This difference was almost entirely due to the presence of high numbers of LB-MBP-specific TCL in MS patients which did not cross-react with LF-MBP and were not present in healthy subjects. LB-MBP may represent a novel antigen worth to be investigated in MS.
机译：对髓磷脂蛋白的自身免疫性T细胞应答被认为与多发性硬化症（MS）的发病机理有关，而髓磷脂碱性蛋白（MBP）是研究最广泛的潜在靶抗原。我们使用两种不同分子形式的蛋白质研究了MS患者和对照中T细胞对MBP的反应：经典的亲水性MBP（无脂质MBP，LF-MBP）和脂质结合的，天然的MBP样制剂以保留与所有髓磷脂脂质（脂质结合的MBP，LB-MBP）结合的分子形式存在。使用LF-MBP或LB-MBP生成短期T细胞系（TCL），并测试它们对体外刺激性抗原的反应性。在MS患者和健康捐献者之间，未检测到对LF-MBP有反应的T细胞培养物的百分比存在差异。相反，MS患者中LB-MBP反应性培养的数量高于对照组。这种差异几乎完全归因于MS患者中大量LB-MBP特异性TCL的存在，这些患者并未与LF-MBP发生交叉反应并且在健康受试者中不存在。 LB-MBP可能代表一种值得在MS中研究的新型抗原。

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