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首页> 外文期刊>Journal of Neuroimmunology: Official Bulletin of the Research Committee on Neuroimmunology of the World Federation of Neurology >Bacillus Calmette-Guerin sequestered in the brain parenchyma escapes immune recognition.
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Bacillus Calmette-Guerin sequestered in the brain parenchyma escapes immune recognition.

机译：隔离在脑实质中的卡介苗芽孢杆菌逃脱了免疫识别。

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We have previously shown that heat-killed bacillus Calmette-Guerin (BCG) injected into the brain parenchyma becomes sequestered behind the blood-brain barrier for months, apparently unrecognised by the immune system (Matyszak and Perry, 1995, 1996a,b). In this paper we have studied T-cell and antibody responses to purified protein derivative (PPD) at different times after intracranial injection of BCG or after the same dose of BCG was injected intradermally. We detected no antibody to PPD in the sera of animals which received intracranial injection, although there was a clear antibody response in the sera of animals injected intradermally, as shown using immunoblot analysis. The skin contact sensitivity to PPD was robust in animals which had received a previous intradermal injection of BCG. 72 h after a PPD injection, the injected site showed many MHC class II + macrophages and T-cells. However, the response in skin following PPD challenge, in animals injected intracranially (i.c.), was comparable with that of naive animals which had received no previous BCG challenge. The skin lesions in animals injected i.c. and in naive animals, were characterised by a small number of MHC class II + cells and rare T-cells. T-cell responses were also studied in an in vitro proliferation assay. The proliferative response was measured for cells isolated from the cervical lymph nodes and the spleen. Cells purified from the spleen and the cervical lymph nodes of animals injected with BCG i.c. showed no specific proliferative response to PPD. The response was comparable to that found in naive, uninjected animals. However, spleen and cervical lymph node cells from animals injected intradermally with BCG showed a significant proliferative response to PPD. These results show that a dose of bacteria injected into the brain parenchyma fails to prime the immune system even though the same dose injected subcutaneously will do so. This response to bacteria in the CNS differs from that previously reported for soluble proteins.
机译：先前我们已经证明，注射入脑实质中的热灭活芽孢杆菌Calmette-Guerin（BCG）在血脑屏障后被隔离了几个月，显然未被免疫系统所识别（Matyszak和Perry，1995，1996a，b）。在本文中，我们研究了颅内注射BCG后或皮内注射相同剂量的BCG后不同时间的T细胞和抗体对纯化的蛋白衍生物（PPD）的反应。如使用免疫印迹分析所示，尽管在皮内注射的动物血清中存在明显的抗体反应，但我们在接受颅内注射的动物血清中未检测到PPD抗体。在先前接受过皮内注射BCG的动物中，对PPD的皮肤接触敏感性很强。 PPD注射后72小时，注射部位显示出许多MHC II类+巨噬细胞和T细胞。但是，在颅内注射（i.c.）的动物中，PPD激发后皮肤中的反应与未接受过BCG激发的幼稚动物相当。经皮下注射的动物皮肤损伤在幼稚动物中，其特征是少量的MHC II类+细胞和稀有T细胞。还在体外增殖测定中研究了T细胞应答。测量从颈淋巴结和脾脏分离的细胞的增殖反应。从注射了BCG的动物的脾脏和颈淋巴结中纯化的细胞。对PPD无特异性增殖反应。该反应与未注射的未注射动物中发现的反应相当。但是，皮内注射BCG的动物的脾脏和颈淋巴结细胞显示出对PPD的明显增殖反应。这些结果表明，即使皮下注射相同剂量的细菌，注入脑实质的一定剂量的细菌也无法启动免疫系统。中枢神经系统对细菌的这种反应与先前报道的对可溶性蛋白质的反应不同。

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