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首页> 外文期刊>Journal of Neuroimmunology: Official Bulletin of the Research Committee on Neuroimmunology of the World Federation of Neurology >Quantification of interleukin-6 mRNA in wallerian degeneration by competitive reverse transcription polymerase chain reaction.
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Quantification of interleukin-6 mRNA in wallerian degeneration by competitive reverse transcription polymerase chain reaction.

机译：定量白细胞变性中白细胞介素6 mRNA的竞争性逆转录聚合酶链反应。

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摘要

Following peripheral nerve injury, cytokines might be critically involved in regulating the cellular response within the lesioned nerve but are frequently difficult to measure due to their low abundancy. Competitive quantitative RT-PCR assays are potentially useful in quantifying cytokine mRNAs in small tissue samples but frequently do not fulfill the theoretical demands on proportional amplification of natural and standard sequences. Using this technique, we constructed standard curves for rat interleukin (IL)-6 mRNA with strictly proportional slopes and shifts when varying natural and standard RNA concentrations. A strong and transient increase in IL-6 mRNA was found in both nerve stumps 12 h after rat sciatic nerve transection, with low expression already in uninjured rat sciatic nerves. IL-6 might be involved in regulating the cellular response to peripheral nerve injury, and standard curves obtained by independent variation of standard and natural RNA are helpful in detecting and controlling experimental variables and pitfalls in competitive RT-PCR assays, thus increasing their reliability and reproducibility.
机译：周围神经损伤后，细胞因子可能参与病变神经内细胞反应的调节，但由于其丰度低而常常难以测量。竞争性定量RT-PCR分析可能可用于定量小组织样品中的细胞因子mRNA，但通常不能满足对自然和标准序列进行比例扩增的理论要求。使用这种技术，我们构建了大鼠白介素（IL）-6 mRNA的标准曲线，当改变天然和标准RNA浓度时，它们具有严格的比例斜率和位移。大鼠坐骨神经横断后12 h，两个神经残端均出现IL-6 mRNA的强而短暂的增加，而未损伤的大鼠坐骨神经中已经有低表达。 IL-6可能参与调节细胞对周围神经损伤的反应，通过标准和天然RNA的独立变异获得的标准曲线有助于检测和控制竞争性RT-PCR分析中的实验变量和缺陷，从而提高其可靠性和可靠性。重现性。

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