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首页> 外文期刊>Journal of Neurophysiology >Dorsal root ganglion neurons express multiple nicotinic acetylcholine receptor subtypes.
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Dorsal root ganglion neurons express multiple nicotinic acetylcholine receptor subtypes.

机译：背根神经节神经元表达多种烟碱型乙酰胆碱受体亚型。

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摘要

Although nicotinic agonists can modulate sensory transmission, particularly nociceptive signaling, remarkably little is known about the functional expression of nicotinic acetylcholine receptors (nAChRs) on primary sensory neurons. We have utilized molecular and electrophysiological techniques to characterize the functional diversity of nAChR expression on mammalian dorsal root ganglion (DRG) neurons. RT-PCR analysis of subunit mRNA in DRG tissue revealed the presence of nAChR subunits alpha2-7 and beta2-beta4. Using whole cell patch-clamp recording and rapid application of nicotinic agonists, four pharmacologically distinct categories of nicotinic responses were identified in cultured DRG neurons. Capacitance measurements were used to divide neurons into populations of large and small cells, and the prevalence of nicotinic responses was compared between groups. Category I (alpha7-like) responses were seen in 77% of large neurons and 32% of small neurons and were antagonized by 10 nM methyllycaconitine citrate (MLA) or or 50 nM alpha-bungarotoxin (alpha-BTX). Category II (alpha3beta4-like) responses were seen in 16% of large neurons and 9% of small neurons and were antagonized by 20 microM mecamylamine but not 10 nM MLA or 1 microM DHbetaE. Category II responses had a higher sensitivity to cytisine than nicotine. Two other types of responses were identified in a much smaller percentage of neurons and were classified as either category III (alpha4beta2-like) or category IV (subtype unknown) responses. Both the alpha7-like and alpha3beta4-like responses could be desensitized by prolonged applications of the analgesic epibatidine.
机译：尽管烟碱激动剂可以调节感觉传递，特别是伤害性信号传导，但关于烟碱乙酰胆碱受体（nAChRs）在初级感觉神经元上的功能性表达知之甚少。我们已经利用分子和电生理技术来表征哺乳动物背根神经节（DRG）神经元上nAChR表达的功能多样性。 DRG组织中亚基mRNA的RT-PCR分析显示nAChR亚基alpha2-7和beta2-beta4的存在。使用全细胞膜片钳记录和烟碱激动剂的快速应用，在培养的DRG神经元中鉴定出四种药理学上不同的烟碱反应类别。电容测量用于将神经元分为大细胞和小细胞，并比较各组之间烟碱反应的发生率。在77％的大神经元和32％的小神经元中观察到I类（alpha7样）反应，并被10 nM柠檬酸甲基卡卡尼汀（MLA）或50 nMα-真菌毒素（alpha-BTX）拮抗。在16％的大神经元和9％的小神经元中观察到II类（alpha3beta4样）响应，并且被20 microM甲基卡敏胺拮抗，但没有10 nM MLA或1 microM DHbetaE拮抗。与烟碱相比，II类反应对胱氨酸的敏感性更高。在较小百分比的神经元中发现了两种其他类型的响应，并将其分类为III类（alpha4beta2样）或IV类（未知亚型）响应。长时间使用止痛药依巴替丁可能会使alpha7-like和alpha3beta4-like反应减敏。

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