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首页> 外文期刊>Biophysical Chemistry: An International Journal Devoted to the Physical Chemistry of Biological Phenomena >DNA triple-helix formation at target sites containing duplex mismatches.
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DNA triple-helix formation at target sites containing duplex mismatches.

机译：在包含双链错配的靶位点上形成DNA三螺旋。

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摘要

We have studied the formation of DNA triple helices at target sites that contain mismatches in the duplex target. Fluorescence melting studies were used to examine a series of parallel triple helices that contain all 64 N.XZ triplet combinations at the centre (where N, X and Z are each of the four natural DNA bases in turn). Similar experiments were also performed with N=bis-amino-U (BAU) (for stable recognition of AT base pairs) and N=S (for recognition of TA inversions). We find that the introduction of a duplex mismatch destabilises the C+.GZ, T.AZ and G.TZ triplets. A similar effect is seen with BAU.AZ triplets. In contrast, other base combinations, based on non-standard triplets such as C.AZ, T.TZ, G.CZ and A.CZ are stabilised by the presence of a duplex mismatch. In each case S binds to sites containing duplex mismatches better than the corresponding Watson-Crick base pairs.
机译：我们已经研究了在双链体靶中含有错配的靶位点上DNA三重螺旋的形成。荧光熔融研究用于检查一系列平行的三重螺旋，这些螺旋在中心包含所有64个N.XZ三重态组合（其中N，X和Z依次是四个天然DNA碱基中的每一个）。还使用N = bis-amino-U（BAU）（用于稳定识别AT碱基对）和N = S（用于TA反转识别）进行了类似的实验。我们发现引入双链失配会破坏C + .GZ，T.AZ和G.TZ三胞胎的稳定性。 BAU.AZ三胞胎也有类似的效果。相反，基于非标准三联体的其他碱基组合（例如C.AZ，T.TZ，G.CZ和A.CZ）通过双链体错配的存在得以稳定。在每种情况下，S与包含双链错配的位点的结合要比相应的Watson-Crick碱基对更好。

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