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首页> 外文期刊>Journal of Neuroscience Methods >Drosophila nerve cord culture: a tool for studying neural development.
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Drosophila nerve cord culture: a tool for studying neural development.

机译:果蝇神经索培养:研究神经发育的工具。

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摘要

The culture of explanted neural tissues has been a useful tool for the study of cellular and molecular neurobiology in vertebrates. We have developed a technique for the culture of explanted ventral nerve cords from Drosophila embryos. We have examined the morphology and dynamic behaviour of the growth cones that extend from these nerve cords, and the effect of calcium deprivation on the bundling of axons that are regenerated from the explanted tissue. This technique offers a unique opportunity to combine in vitro techniques for neuronal cell culture with the powerful techniques of genetic analysis that are available with Drosophila.
机译:外植神经组织的培养已成为研究脊椎动物细胞和分子神经生物学的有用工具。我们已经开发出一种用于培养果蝇胚胎腹侧神经索的技术。我们已经检查了从这些神经索延伸的生长锥的形态和动态行为,以及缺钙对从外植组织再生的轴突束的影响。该技术提供了独特的机会,可以将用于神经元细胞培养的体外技术与果蝇可获得的强大的遗传分析技术相结合。

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