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首页> 外文期刊>Biophysical Chemistry: An International Journal Devoted to the Physical Chemistry of Biological Phenomena >AFM imaging and theoretical modeling studies of sequence-dependent nucleosome positioning
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AFM imaging and theoretical modeling studies of sequence-dependent nucleosome positioning

机译：依赖序列的核小体定位的AFM成像和理论建模研究

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摘要

Telomeric chromatin has different features with respect to bulk chromatin, since nucleosomal repeat along the chain is unusually short. We studied the role of telomeric DNA sequences on nucleosomal spacing in a model system. Nucleosomal arrays, assembled on a 1500-bp-long human telomeric DNA and on a DNA fragment containing 8 copies of the 601 strong nucleosome positioning sequence, have been studied at the single molecule level, by atomic force microscopy imaging. Random nucleosome positioning was found in the case of human telomeric DNA. On the contrary, nucleosome positioning on 601 DNA is characterized by preferential positions of nucleosome dyad axis each 200 bp. The AFM-derived nucleosome organization is in satisfactory agreement with that predicted by theoretical modeling, based on sequence-dependent DNA curvature and flexibility. The reported results show that DNA sequence has a main role, not only in mononucleosome thermodynamic stability, but also in the organization of nucleosomal arrays.
机译：端粒染色质相对于整体染色质具有不同的特征，因为沿链的核小体重复异常短。我们研究了端粒DNA序列在模型系统中对核小体间隔的作用。已经通过原子力显微镜成像研究了在单个分子水平上组装在1500 bp长的人类端粒DNA和包含601个强核小体定位序列的8个拷贝的DNA片段上的核糖体阵列。对于人类端粒DNA，发现了随机的核小体定位。相反，核小体在601 DNA上的定位特征是核小体dyad轴的优先位置各为200 bp。 AFM衍生的核小体组织与基于序列依赖的DNA曲率和柔韧性的理论建模预测的组织令人满意。报道的结果表明，DNA序列不仅在单核小体的热力学稳定性中，而且在核小体阵列的组织中都具有主要作用。

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