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首页> 外文期刊>Journal of Neuroscience Methods >Gene expression profiles of rat olfactory bulb at developmental stage.
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Gene expression profiles of rat olfactory bulb at developmental stage.

机译：大鼠嗅球发育阶段的基因表达谱。

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摘要

Microarray analysis may be a useful tool to identify some candidate genes related to the development of olfactory bulbs. In the present study, gene expression profiles of olfactory bulbs from postnatal day 1 (P1) rats and postnatal day 35 (P35) rats were analyzed by oligonucleotide-microarray and expression levels of some selected genes were also confirmed by RT-PCR and in situ hybridization. 9146 genes were commonly identified in six microarray chips. Among these genes, 76 were up-regulated and 130 were down-regulated three-folds or more at P1 olfactory bulbs. Out of these 76 up-regulated genes, 24 genes were annotated based on the NCBI database of reference sequences and expression levels of these 24 genes were confirmed by RT-PCR. Among them, 2 interesting genes (neurogenic differentiation 1 and retinoid acid receptor alpha) were localized in the P1 olfactory bulb by the use of in situ hybridization technique. Our results may provide basic information to identify genes associated with functional growth of olfactory bulbs.
机译：微阵列分析可能是鉴定一些与嗅球发育有关的候选基因的有用工具。在本研究中，通过寡核苷酸微阵列分析了出生后第1天（P1）大鼠和出生后第35天（P35）大鼠嗅球的基因表达谱，并通过RT-PCR和原位证实了某些选定基因的表达水平杂交。通常在六个微阵列芯片中鉴定出9146个基因。在这些基因中，P1嗅球上调了76倍，下调了130倍，三倍以上。在这76个上调基因中，基于NCBI参考序列数据库对24个基因进行了注释，并通过RT-PCR确认了这24个基因的表达水平。其中，通过使用原位杂交技术，在P1嗅球中定位了2个有趣的基因（神经分化1和类维生素A受体α）。我们的结果可能提供基本信息，以鉴定与嗅球功能生长相关的基因。

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