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首页> 外文期刊>Journal of Neuroscience Methods >Mitochondrial function and cerebral blood flow variable responses to middle cerebral artery occlusion.
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Mitochondrial function and cerebral blood flow variable responses to middle cerebral artery occlusion.

机译：线粒体功能和脑血流变量对大脑中动脉闭塞的反应。

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Middle cerebral artery occlusion (MCAO), which leads to focal cerebral ischemia, serves as an experimental model for brain stroke. There is a large variation in protocols and techniques using the MCAO model, which may affect the outcomes seen in different studies. The current work presents and compares the diverse responses in mitochondrial NADH and cerebral blood flow (CBF) following focal ischemia induced by the MCAO technique. Ninety-six Wistar rats underwent focal cerebral ischemia by MCAO, and monitored in the core and the penumbra using a unique Multi-Site-Multi-Parametric (MSMP) system, which measures mitochondrial NADH using the fluorometric technique, and CBF using laser Doppler flowmetry (LDF). Following MCAO, 58% of the experiments yielded expected responses, namely a decrease in CBF and an increase in NADH. However, 42% of the experiments showed six other profiles of responses, in which CBF, NADH and tissue reflectance (Ref) responded differently. These profiles included: ischemia without reperfusion, death following reperfusion, minor responses in parameters during ischemia, CBF elevation in the penumbra following MCAO, spontaneous early reperfusion and late reperfusion. These results demonstrate that MCAO is a complex model, which may lead to different responses other than the common expected outcomes, i.e. mitochondrial damage and reduced blood flow in both core and penumbra. The MSMP monitoring system may serve as an important tool in early diagnosis of successful focal cerebral ischemia, reducing the percentage of unsuccessful experiments.
机译：导致局灶性脑缺血的大脑中动脉闭塞（MCAO）作为脑卒中的实验模型。使用MCAO模型的协议和技术存在很大差异，这可能会影响不同研究中看到的结果。当前的工作提出并比较了由MCAO技术引起的局灶性缺血后线粒体NADH和脑血流量（CBF）的不同反应。九十六只Wistar大鼠通过MCAO进行了局灶性脑缺血，并使用独特的多站点多参数（MSMP）系统在核心和半影中进行了监测，该系统使用荧光技术测量线粒体NADH，并使用激光多普勒血流仪测量CBF （LDF）。 MCAO之后，58％的实验产生了预期的响应，即CBF降低和NADH升高。但是，有42％的实验显示了其他六种反应曲线，其中CBF，NADH和组织反射率（Ref）的反应不同。这些特征包括：没有再灌注的局部缺血，再灌注后的死亡，局部缺血期间参数的较小反应，MCAO后半影中的CBF升高，自发的早期再灌注和晚期再灌注。这些结果表明MCAO是一个复杂的模型，除了常见的预期结果外，它可能导致不同的反应，即线粒体损伤和核心和半影的血流减少。 MSMP监测系统可以作为早期成功诊断局灶性脑缺血的重要工具，从而减少实验失败的百分比。

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