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首页> 外文期刊>Journal of Neuroscience Methods >Extensive scarring induced by chronic intrathecal tubing augmented cord tissue damage and worsened functional recovery after rat spinal cord injury.
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Extensive scarring induced by chronic intrathecal tubing augmented cord tissue damage and worsened functional recovery after rat spinal cord injury.

机译：慢性鞘内导管引起的广泛疤痕增加了脊髓组织损伤后的脊髓组织损伤，并使功能恢复恶化。

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摘要

Intrathecal infusion has been widely used to directly deliver drugs or neurotrophins to a lesion site following spinal cord injury. Evidence shows that intrathecal infusion is efficient for 7 days but is markedly reduced after 14 days, due to time dependent occlusion. In addition, extensive fibrotic scarring is commonly observed with intrathecal infusion. These anomalies need to be clearly elucidated in histology. In the present study, all adult Long-Evans rats received a 25 mm contusion injury on spinal cord T10 produced using the NYU impactor device. Immediately after injury, catheter tubing with an outer diameter of 0.38 mm was inserted through a small dural opening at L3 into the subdural space with the tubing tip positioned near the injury site. The tubing was connected to an Alzet mini pump, which was filled with saline solution and was placed subcutaneously. Injured rats without tubing served as control. Rats were behaviorally tested for 6 weeks using the BBB locomotor rating scale and histologically assessed for tissue scarring. Six weeks later, we found that the intrathecal tubing caused extensive scarring and inflammation, related to neutrophils, macrophages and plasma cells. The tubing's tip was occluded by scar tissue and inflammatory cells. The scar tissue surrounding the tubing consists of 20-70 layers of fibroblasts and densely compacted collagen fibers, seriously compressing and damaging the cord tissue. BBB scores of rats with intrathecal tubing were significantly lower than control rats (p<0.01) from 2 weeks after injury, implying serious impairment of functional recovery caused by the scarring.
机译：鞘内灌注已被广泛用于在脊髓损伤后将药物或神经营养蛋白直接递送至病变部位。证据表明鞘内输注有效7天，但由于时间依赖性闭塞，在14天后明显减少。另外，鞘内灌注通常观察到广泛的纤维化瘢痕形成。这些异常需要在组织学中清楚地阐明。在本研究中，所有成年的Long-Evans大鼠在使用NYU撞击器装置产生的脊髓T10上遭受了25 mm挫伤。受伤后，立即将外径为0.38 mm的导管通过L3处的硬膜小孔插入硬膜下腔，同时将导管尖端置于受伤部位附近。将管子连接到装有盐溶液并皮下放置的Alzet微型泵。不用管的受伤大鼠作为对照。使用BBB运动评分量表对大鼠进行了6周的行为测试，并组织学评估了组织瘢痕形成。六周后，我们发现鞘内导管引起广泛的瘢痕形成和炎症，与中性粒细胞，巨噬细胞和浆细胞有关。疤痕组织和炎性细胞阻塞了管的尖端。管道周围的疤痕组织由20-70层成纤维细胞和致密的胶原纤维组成，严重压迫和损坏了脐带组织。鞘内输卵管大鼠的BBB评分从受伤后2周开始显​​着低于对照组（p <0.01），这表明瘢痕形成会严重损害功能恢复。

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