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首页> 外文期刊>Journal of Neuroscience Methods >A modified Hargreaves' method for assessing threshold temperatures for heat nociception
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A modified Hargreaves' method for assessing threshold temperatures for heat nociception

机译：一种改进的Hargreaves方法，用于评估热伤害感受的阈值温度

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This study describes a modified Hargreaves' method for assessing paw withdrawal threshold temperatures for heat (PWT-H) nociception in the hind paws of rats. This method utilises radiant heat to maintain controlled lamp temperatures (CLTs) on a glass floor beneath the rat hind paw. An ascending series of CLTs were applied for 10. s, with 5-10. min intervals between applications, until characteristic withdrawal behaviour was observed or a cutoff CLT was reached. The average plantar epicutaneous temperatures measured from anaesthetised rats corresponding to CLTs and withdrawal latencies were used for determining PWT-H. The mean PWT-H in 2-month-old (mo) na?ve Sprague-Dawley rats ( n= 38) was 47.6. ±. 0.2. °C, which is comparable to the noxious threshold temperature for human glabrous skin (46.5. ±. 0.5. °C). The PWT-H was consistent between trials and daily assessments over four consecutive days. No significant differences were observed between the PWT-H in 2-, 6- to 8-, and >24-mo F344 rats, but the PWT-H in 1-mo rats was significantly reduced. Three hours following plantar incision, the PWT-H decreased to 37.5. ±. 0.2. °C, consistent with previous observations of C-fibre afferents from incised glabrous skin firing at 36.7. ±. 3.6. °C. Parallel testing, using the current method and an electronic von Frey device, illustrated similar degrees of incision-induced hyperalgesia, gradual improvements in hyperalgesia, and reversals induced through morphine and gabapentin. In conclusion, the present method facilitates a comparison of PWT-H using electrophysiological and human psychophysical studies involving thermosensation, and as a behavioural assay identical to von Frey testing, this method also measures the threshold for nociception.
机译：这项研究描述了一种改良的Hargreaves方法，用于评估大鼠后爪热痛（PWT-H）伤害的爪缩回阈温度。该方法利用辐射热在老鼠后爪下方的玻璃地板上维持可控的灯温度（CLT）。一系列CLT升序应用10 s，5-10。间隔两次，直到观察到特征性的戒断行为或达到临界CLT。从麻醉大鼠测得的平均足底表皮温度对应于CLTs和戒断潜伏期，用于确定PWT-H。 2月龄（运动）纯Sprague-Dawley大鼠（n = 38）的平均PWT-H为47.6。 ±。 0.2。 °C，可与人体无毛皮肤的有害阈值温度相比（46.5。±。0.5。°C）。 PWT-H在连续四天的试验和每日评估之间保持一致。在2个月，6个月，8个月和> 24个月F344大鼠中，PWT-H之间没有观察到显着差异，但1个月大鼠中的PWT-H明显降低。足底切开三小时后，PWT-H降至37.5。 ±。 0.2。 °C，与先前观察到的36.7切开的无毛皮肤灼烧C纤维传入的观察结果一致。 ±。 3.6。 ℃。使用当前方法和电子von Frey装置进行的并行测试显示了类似程度的切口诱发的痛觉过敏，痛觉过敏的逐步改善以及通过吗啡和加巴喷丁引起的逆转。总之，本方法便于使用涉及热感觉的电生理学和人类心理物理研究对PWT-H进行比较，并且作为与von Frey测试相同的行为分析，该方法还可以测量伤害感受的阈值。

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