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首页> 外文期刊>Journal of ocular pharmacology and therapeutics: The official journal of the Association for Ocular Pharmacology and Therapeutics >Evaluation of differential toxicity of varying doses of bevacizumab on retinal ganglion cells, retinal pigment epithelial cells, and vascular endothelial growth factor-enriched choroidal endothelial cells.
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Evaluation of differential toxicity of varying doses of bevacizumab on retinal ganglion cells, retinal pigment epithelial cells, and vascular endothelial growth factor-enriched choroidal endothelial cells.

机译：评价不同剂量的贝伐单抗对视网膜神经节细胞，视网膜色素上皮细胞和富含血管内皮生长因子的脉络膜内皮细胞的毒性差异。

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摘要

PURPOSE: To evaluate in vitro the effects of bevacizumab, an anti-vascular endothelial growth factor (VEGF) antibody, on retinal pigment epithelial cells (RPE) and retinal ganglion cells (RGC), at doses that were inhibitory to VEGF-enriched choroidal endothelial cells (CEC). METHODS: Monkey CEC (RF6A), human RPE cells (ARPE-19), and rat RGC (RGC-5) were exposed for 24 h to increasing doses of bevacizumab. Cell numbers were quantified with WST-1 assay. Cell death was assessed using propidium iodide (PI) staining via flow cytometry and fluorescent microscopy. RESULTS: Bevacizumab was inhibitory to RF6A at 2.0 mg/mL (P < 0.005). No effect on cell viability was noted on ARPE-19 and RGC-5 cell lines at this particular dose of bevacizumab. These results were supported by fluorescent microscopy of PI-stained cells. CONCLUSIONS: VEGF-stimulated proliferation of CEC was inhibited by bevacizumab. Bevacizumab was not cytotoxic to human RPE and rat RGC in vitro at a dose that is inhibitory to monkey CEC.
机译：目的：在体外评估贝伐单抗（一种抗血管内皮生长因子（VEGF）抗体）对视网膜色素上皮细胞（RPE）和视网膜神经节细胞（RGC）的抑制作用，该剂量可抑制富含VEGF的脉络膜内皮单元（CEC）。方法：将猴子CEC（RF6A），人RPE细胞（ARPE-19）和大鼠RGC（RGC-5）暴露于增加剂量的贝伐单抗24 h。用WST-1测定法定量细胞数。通过流式细胞术和荧光显微镜术使用碘化丙啶（PI）染色评估细胞死亡。结果：贝伐单抗以2.0 mg / mL抑制RF6A（P <0.005）。在该特定剂量的贝伐单抗下，未观察到对ARPE-19和RGC-5细胞系的细胞活力有影响。这些结果得到PI染色细胞的荧光显微镜检查的支持。结论：贝伐单抗可抑制VEGF刺激的CEC增殖。贝伐单抗在体外以抑制猴CEC的剂量对人RPE和大鼠RGC无细胞毒性。

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