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首页> 外文期刊>Journal of Nutritional Science and Vitaminology >Polypyrimidine tract-binding protein is involved in regulation of albumin synthesis in response to food intake.
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Polypyrimidine tract-binding protein is involved in regulation of albumin synthesis in response to food intake.

机译：多嘧啶束结合蛋白响应于食物摄入而参与白蛋白合成的调节。

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Our recent study demonstrates that polypyrimidine tract-binding protein (PTB), which is a sequence specific RNA-binding protein, attenuates albumin synthesis in a cell-free translation system. In this study, the effects of food intake on regulation of albumin synthesis through binding of PTB to albumin messenger RNA (mRNA) were investigated. Rats were divided into 1 of 3 groups: fed; fasted for 36 h; or fasted for 36 h and then refed for 24 h. No significant differences in albumin mRNA levels were found among fed, fasted and refed rats. However, a decrease in the proportion of albumin mRNA associated with polysomes was identified in fasted rats. Furthermore, UV-cross linking analysis demonstrated that levels of albumin mRNA-PTB complex were increased in liver extracts from fasted rats. No significant differences in PTB levels in liver homogenate were found among the experimental groups. However, PTB level in the cytoplasmic fraction was higher in fasted rats than in fed rats. In refed rats, PTB level in the cytoplasmic fraction returned to a level comparable to that in fed rats, but was inhibited by treatment with rapamycin, a mammalian target of rapamycin (mTOR) inhibitor. These results suggest that localization of PTB is regulated by food intake through mTOR signaling, and alterations in level of albumin mRNA-PTB complex play a role in mediating the effects of food intake on albumin synthesis in the rat liver.
机译：我们最近的研究表明，聚嘧啶束结合蛋白（PTB）是一种序列特异性RNA结合蛋白，可在无细胞翻译系统中减弱白蛋白的合成。在这项研究中，研究了食物摄入通过PTB与白蛋白信使RNA（mRNA）的结合对白蛋白合成的调节作用。将大鼠分为3组中的1组：进食;进食。禁食36小时;或禁食36小时，然后禁食24小时。在喂食，禁食和摄食的大鼠中，白蛋白mRNA水平没有显着差异。然而，在禁食的大鼠中发现与多核糖体相关的白蛋白mRNA的比例降低。此外，紫外线交联分析表明，禁食大鼠肝脏提取物中白蛋白mRNA-PTB复合物的水平增加。在实验组之间，肝脏匀浆中PTB水平没有显着差异。然而，禁食大鼠的细胞质级分中的PTB水平高于进食大鼠。在参照大鼠中，细胞质级分中的PTB水平恢复到与进食大鼠中相当的水平，但被雷帕霉素（雷帕霉素（mTOR）抑制剂的哺乳动物靶标）治疗抑制。这些结果表明，PTB的定位受食物通过mTOR信号传导的调节，并且白蛋白mRNA-PTB复合物水平的改变在介导食物摄入对大鼠肝脏白蛋白合成的影响中起作用。

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