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首页> 外文期刊>Journal of orthopaedic research >Cell traffic between donor and recipient following rat limb allograft.
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Cell traffic between donor and recipient following rat limb allograft.

机译：大鼠肢体同种异体移植后供体和受体之间的细胞运输。

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摘要

Although cell traffic from the graft into the recipient and from the recipient into the graft had been noticed in allogeneic organ transplantation, little is known following whole-limb allografting. This study was conducted to define cell migration between donor and recipient. Sixty-seven vascularized hind limb allotransplantations were performed in rat sex-mismatched pairs and the recipient animals were treated with FK506 immunosuppression. The ratio of donor and recipient cells was evaluated by semi-quantitative PCR using the specific primers of the Y-chromosome. Allografted limbs had no rejection episode until the final assessment. The male recipient cells were detected in female limb grafts not at 1 week but at 48 weeks after transplantation. The male donor cells were detected in the humerus and tibia in the female recipient but not in the gastrocnemius muscle and leg skin. Our results demonstrated that recipient-derived cells gradually migrated into the grafted bone, muscle and skin cells with theduration of time. Donor-derived cells migrated into the healthy bones but not into the healthy muscle and skin. Because active regeneration occurs in the grafted limb to compensate graft damage secondary to ischemia and operative intervention, recipient-derived cells may mediate a muscular and dermo-epidermal renewal.
机译：尽管在同种异体器官移植中已经注意到从移植物到受体以及从受体到移植物的细胞运输，但是在全肢同种异体移植之后知之甚少。进行该研究以定义供体和受体之间的细胞迁移。在大鼠性别不匹配的配对中进行了67例血管化后肢同种异体移植，并用FK506免疫抑制治疗了接受动物。使用Y染色体的特异性引物通过半定量PCR评估供体和受体细胞的比例。同种异体移植四肢在最终评估前无排斥反应。移植后第1周而非第48周在雌性肢体移植物中检测到雄性受体细胞。在女性接受者的肱骨和胫骨中检测到男性供体细胞，但在腓肠肌和腿部皮肤中未检测到。我们的结果表明，随着时间的推移，受体来源的细胞逐渐迁移到移植的骨，肌肉和皮肤细胞中。供体来源的细胞迁移到健康的骨骼中，但没有迁移到健康的肌肉和皮肤中。由于活动的再生发生在移植的肢体中，以补偿继发于缺血和手术干预的移植物损害，因此受体衍生的细胞可能介导肌肉和真皮-表皮的更新。

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