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首页> 外文期刊>Journal of pharmaceutical sciences. >Evaluation of fluorescent probe surface intensities as an indicator of transdermal permeant distributions using wide-area two-photon fluorescence microscopy.
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Evaluation of fluorescent probe surface intensities as an indicator of transdermal permeant distributions using wide-area two-photon fluorescence microscopy.

机译：使用广域两光子荧光显微镜评估荧光探针表面强度，作为透皮渗透剂分布的指标。

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The application of high-speed two-photon fluorescence microscopy (HTPM) to examine transdermal transport processes has enabled the noninvasive visualization of permeant spatial distributions over a larger, more clinically relevant wide area of the skin. Earlier studies demonstrated that the transdermal fluorescent probe distribution over a 2 x 2 mm skin area was well represented by a significantly reduced sampling of the 400 microscale skin sites (100 x 100 microm) constituting the wide area. In the present study, the 400 microscale skin sites are considered individually, and the site-to-site variability in permeant distributions is used as a model to reflect the range in experimentally measured skin permeabilities resulting from the inherent stratum corneum structural heterogeneity. The correlation established between the permeant surface intensity and the corresponding permeant intensity gradient at each skin site provides an indication of the potential for screening transdermal permeant distributions solely based on the evaluation of microscale permeant surface intensities. The strong linear correlation between the intensity gradient and the surface intensity for the hydrophilic model permeant, sulforhodamine B, demonstrated that surface intensities provide a robust indicator of the corresponding transdermal probe distributions at the microscale. For the hydrophobic model permeant, rhodamine B hexyl ester, however, weak correlations were observed between these two parameters. This result suggests that the stratum corneum microscale surface intensity does not validly capture the corresponding intensity gradients for the entire range of skin permeabilities typically encountered as a result of the inherent stratum corneum heterogeneity.
机译：高速双光子荧光显微镜（HTPM）的应用检查透皮运输过程，使得能够在更大，更临床相关的大面积皮肤上无创地观察渗透性空间分布。较早的研究表明，透皮荧光探针在2 x 2 mm皮肤区域上的分布可以明显地减少构成宽区域的400个微尺度皮肤部位（100 x 100微米）的采样。在本研究中，单独考虑了400个微尺度的皮肤部位，渗透位点分布的位点间差异被用作模型，以反映由固有角质层结构异质性导致的实验测量的皮肤渗透位的范围。在每个皮肤部位的渗透表面强度和相应的渗透强度梯度之间建立的相关性提供了仅基于微尺度渗透表面强度评估筛选透皮渗透分布的潜力的指示。亲水模型渗透物磺基若丹明B的强度梯度和表面强度之间的强线性相关性表明，表面强度为相应的透皮探针在微观尺度上的分布提供了可靠的指标。对于疏水模型渗透物，若丹明B己基酯，然而，这两个参数之间观察到弱的相关性。该结果表明，角质层微观尺度的表面强度不能有效捕获由于固有的角质层异质性而通常遇到的整个皮肤渗透性范围的相应强度梯度。

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