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首页> 外文期刊>Journal of pharmaceutical sciences. >Characterization of antigens adsorbed to anionic PLG microparticles by XPS and TOF-SIMS.
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Characterization of antigens adsorbed to anionic PLG microparticles by XPS and TOF-SIMS.

机译：XPS和TOF-SIMS表征吸附到阴离子PLG微粒上的抗原。

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The chemical composition of the surface of anionic PLG microparticles before and after adsorption of vaccine antigens was measured using X-ray photoelectron spectroscopy (XPS) and time-of-flight secondary ion mass spectrometry (TOF-SIMS). The interfacial distributions of components will reflect underlying interactions that govern properties such as adsorption, release, and stability of proteins in microparticle vaccine delivery systems. Poly(lactide-co-glycolide) microparticles were prepared by a w/o/w emulsification method in the presence of the anionic surfactant dioctyl sodium sulfosuccinate (DSS). Ovalbumin, lysozyme, a recombinant HIV envelope glyocoprotein and a Neisseria meningitidis B protein were adsorbed to the PLG microparticles, with XPS and time-of-flight secondary mass used to analyze elemental and molecular distributions of components of the surface of lyophilized products. Protein (antigen) binding to PLG microparticles was measured directly by distinct elemental and molecular spectroscopic signatures consistent with amino acids and excipient species. The surface sensitive composition of proteins also included counter ions that support the importance of electrostatic interactions being crucial in the mechanism of adsorptions. The protein binding capacity was consistent with the available surface area and the interpretation of previous electron and atomic force microscope images strengthened by the quantification possible by XPS and the qualitative identification possible with TOF-SIMS. Protein antigens were detected and quantified on the surface of anionic PLG microparticles with varying degrees of efficiency under different adsorption conditions such as surfactant level, pH, and ionic strength. Observable changes in elemental and molecular composition suggest an efficient electrostatic interaction creating a composite surface layer that mediates antigen binding and release.
机译：使用X射线光电子能谱（XPS）和飞行时间二次离子质谱（TOF-SIMS）测量了疫苗抗原吸附前后阴离子PLG微粒表面的化学组成。组分的界面分布将反映潜在的相互作用，这些相互作用决定着微粒疫苗输送系统中蛋白质的吸附，释放和稳定性等特性。在阴离子表面活性剂磺基琥珀酸二辛基钠二辛酯的存在下，通过w / o / w乳化法制备了聚丙交酯-共-乙交酯共聚物微粒。将卵清蛋白，溶菌酶，重组HIV包膜糖蛋白和脑膜炎奈瑟氏球菌B蛋白吸附到PLG微粒上，并用XPS和飞行时间二次质量分析冻干产品表面成分的元素和分子分布。通过与氨基酸和赋形剂种类一致的独特元素和分子光谱特征直接测量与PLG微粒结合的蛋白质（抗原）。蛋白质的表面敏感成分还包括抗衡离子，这些离子支持静电相互作用的重要性，而静电相互作用对吸附机理至关重要。蛋白质的结合能力与可用的表面积相一致，并且通过XPS的定量分析和TOF-SIMS的定性鉴定可以加强对以前的电子和原子力显微镜图像的解释。在不同的吸附条件（例如表面活性剂水平，pH和离子强度）下，以不同的效率程度检测和定量蛋白质PLN阴离子表面上的蛋白质抗原。元素和分子组成的可观察到的变化表明有效的静电相互作用产生介导抗原结合和释放的复合表面层。

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