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首页> 外文期刊>Journal of Plant Physiology >Preferential inhibition of the lycopene epsilon-cyclase by the substituted triethylamine compound MPTA in higher plants
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Preferential inhibition of the lycopene epsilon-cyclase by the substituted triethylamine compound MPTA in higher plants

机译：取代的三乙胺化合物MPTA优先抑制番茄红素ε-环化酶

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摘要

In addition to the usual complement of carotenoids found in the plant leaf tissues, lettuce (Lactuca sativa), unusually, possesses large amounts of the diol lactucaxanthin. This carotenoid possesses two epsilon-end-groups and its presence provides a good model in which to study the effects of the substituted triethylamine compound 2-(4-methylphenoxy)triethylamine (MPTA) on the cyclisation of beta- and epsilon-end-groups during the biosynthesis of carotenoids. Treatment with 10 or 20microM MPTA significantly reduced levels of both beta-carotene and neoxanthin (up to 18-fold), whilst levels of violaxanthin and lutein were less affected (4-fold reduction). In contrast, levels of lactucaxanthin were not reduced even at the highest inhibitor concentration, and at 10microM MPTA levels of this xanthophyll doubled. The pigment stoichiometry of the bulk light-harvesting complex (LHCIIb) isolated from treated plants shows that lactucaxanthin successfully substituted for lutein and neoxanthin in two of the xanthophyll binding sites, namely L2 and N1. Inhibition of cyclisation was accompanied by the accumulation of lycopene and trace amounts of delta-carotene and a number of oxygenated derivatives of these precursors. Two forms of mono-hydroxy lycopene were identified together with mono-epoxy delta-carotene.
机译：除了通常在植物叶片组织中发现的类胡萝卜素外，生菜（Lactuca sativa）通常还含有大量的二醇乳糖黄嘌呤。该类胡萝卜素具有两个ε端基，其存在为研究取代的三乙胺化合物2-（4-甲基苯氧基）三乙胺（MPTA）对β-和ε端基环化的影响提供了一个很好的模型。在类胡萝卜素的生物合成过程中。用10或20 microM MPTA处理可显着降低β-胡萝卜素和新黄嘌呤的含量（最高18倍），而紫黄质和叶黄素的含量受到的影响较小（降低4倍）。相反，即使在最高抑制剂浓度下，乳黄嘌呤的水平也不会降低，并且在10microM MPTA下，该叶黄素水平也翻了一番。从处理过的植物中分离的大量采光复合物（LHCIIb）的色素化学计量显示，在两个叶黄素结合位点L2和N1中，乳黄嘌呤成功地取代了叶黄素和新黄质。环化的抑制伴随着番茄红素的积累和痕量的δ-胡萝卜素以及这些前体的许多氧化衍生物。鉴定了两种形式的单羟基番茄红素和单环氧δ-胡萝卜素。

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