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首页> 外文期刊>Journal of Pathology: Journal of the Pathological Society of Great Britain and Ireland >Search for transmembrane protein in gastric cancer by the Escherichia coli ampicillin secretion trap: expression of DSC2 in gastric cancer with intestinal phenotype
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Search for transmembrane protein in gastric cancer by the Escherichia coli ampicillin secretion trap: expression of DSC2 in gastric cancer with intestinal phenotype

机译：大肠杆菌氨苄青霉素分泌陷阱在胃癌中寻找跨膜蛋白：DSC2在肠表型胃癌中的表达

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摘要

Gastric cancer (GC) is one of the most common malignancies worldwide. Genes expressed only in cancer tissue, and especially on the cell membrane, will be useful molecular markers for diagnosis and may also be good therapeutic targets. To identify genes that encode transmembrane proteins present in GC, we generated Escherichia coli ampicillin secretion trap (CAST) libraries from two GC cell lines and normal stomach. By sequencing 4320 colonies from CAST libraries, we identified 30 candidate genes that encode transmembrane proteins present in GC. Quantitative reverse transcription-polymerase chain reaction analysis of these candidates revealed that ZDHHC14, BST2, DRAM2, and DSC2 were expressed much more highly in GC than in 14 kinds of normal tissues. Among these, DSC2 encodes desmocollin 2, which is one of three known desmocollins. Immunohistochemical analysis demonstrated that 22 (28?/o) of 80 GC cases were positive for desmocollin 2, and desmocollin 2 expression was observed frequently in GC with the intestinal mucin phenotype. Furthermore, desmocollin 2 expression was correlated with CDX2 expression. These results suggest that expression of desmocollin 2, induced by CDX2, may be a key regulator for GC with the intestinal mucin phenotype. Our results provide a list of genes that have high potential as a diagnostic and therapeutic target for GC.
机译：胃癌（GC）是全球最常见的恶性肿瘤之一。仅在癌组织中，尤其是在细胞膜上表达的基因，将成为诊断有用的分子标记，也可能是良好的治疗靶标。为了鉴定编码GC中存在的跨膜蛋白的基因，我们从两个GC细胞系和正常胃中生成了大肠杆菌氨苄青霉素分泌陷阱（CAST）库。通过对CAST文库中的4320个菌落进行测序，我们鉴定了30个编码GC中存在的跨膜蛋白的候选基因。对这些候选基因的定量逆转录-聚合酶链反应分析表明，与14种正常组织相比，GC中ZDHHC14，BST2，DRAM2和DSC2的表达高得多。其中，DSC2编码desmocollin 2，它是三​​种已知的desmocollins之一。免疫组织化学分析显示80例胃癌患者中有22例（28？/ o）的desmocollin 2呈阳性，在具有肠粘蛋白表型的GC中经常观察到desmocollin 2的表达。此外，desmocollin 2表达与CDX2表达相关。这些结果表明，由CDX2诱导的desmocollin 2的表达可能是具有肠粘蛋白表型的GC的关键调节因子。我们的结果提供了具有高潜力作为GC诊断和治疗靶标的基因清单。

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