• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Pathobiology: journal of immunopathology, molecular and cellular biology >The search for secreted proteins in prostate cancer by the Escherichia coli ampicillin secretion trap: expression of NBL1 is highly restricted to the prostate and is related to cancer progression.
【24h】

The search for secreted proteins in prostate cancer by the Escherichia coli ampicillin secretion trap: expression of NBL1 is highly restricted to the prostate and is related to cancer progression.

机译：通过大肠杆菌氨苄青霉素分泌陷阱来寻找前列腺癌中的分泌蛋白：NBL1的表达高度局限于前列腺，并且与癌症进展有关。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Genes expressed only in cancer tissue or specific organs will be useful molecular markers. To identify genes that encode secreted proteins present in prostate cancer (PCa), we generated Escherichia coli ampicillin secretion trap (CAST) libraries from PCa and normal prostate (NP).We identified 15 candidate genes that encode secreted proteins present in PCa and NP. Quantitative RT-PCR analysis revealed that MSMB, NBL1 and AZGP1 were expressed with much higher specificity in PCa and NP than in 14 other kinds of normal tissue. We focused on NBL1, which was originally identified as a putative tumor suppressor gene. Western blot analysis revealed that NBL1 protein was highly expressed in both cell lysate and culture media of the DU145 PCa cell line. Immunohistochemical analysis showed that NBL1 expression was highly detected in and restricted to NP and PCa and was significantly down-regulated in PCa. NBL1 expression was significantly reduced according to the tumor stage, Gleason grade and preoperative prostate-specific antigen (PSA) value.NBL1 is a secreted protein that is highly restricted to the prostate. Underexpression of NBL1 correlated with PCa progression. NBL1 might be a candidate tumor marker for PCa in addition to PSA.
机译：仅在癌组织或特定器官中表达的基因将是有用的分子标记。为了鉴定编码前列腺癌（PCa）中分泌蛋白的基因，我们从PCa和正常前列腺（NP）中产生了大肠杆菌氨苄青霉素分泌陷阱（CAST）库，我们鉴定了15种编码PCa和NP中分泌蛋白的候选基因。定量RT-PCR分析显示，MSMB，NBL1和AZGP1在PCa和NP中的表达比在其他14种正常组织中高得多。我们专注于NBL1，它最初被认为是推定的肿瘤抑制基因。蛋白质印迹分析表明，NBL1蛋白在DU145 PCa细胞系的细胞裂解液和培养基中均高表达。免疫组织化学分析显示，NBL1表达在NP和PCa中被高度检测到且受其限制，并且在PCa中被显着下调。 NBL1的表达根据肿瘤的分期，格里森分级和术前前列腺特异性抗原（PSA）的值而显着降低。NBL1是一种高度限制前列腺的分泌蛋白。 NBL1的表达不足与PCa进展相关。除了PSA，NBL1可能是PCa的候选肿瘤标志物。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号