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首页> 外文期刊>Clinical immunology: The official journal of the Clinical Immunology Society >Functional analysis of human memory B-cell subpopulations: IgD(+)CD27(+) B cells are crucial in secondary immune response by producing high affinity IgM.
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Functional analysis of human memory B-cell subpopulations: IgD(+)CD27(+) B cells are crucial in secondary immune response by producing high affinity IgM.

机译：人类记忆B细胞亚群的功能分析：IgD（+）CD27（+）B细胞通过产生高亲和力IgM在继发免疫反应中至关重要。

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摘要

The number of memory B cells in peripheral blood has been assayed in various diseases by using CD27 as a memory B-cell marker. However, the defining differences of characteristic and function between the two memory B-cell subpopulations separated by immunoglobulin (Ig)D expression remain to be clearly elucidated. We analyzed here IgD(+)CD27(+) B cells (circulating B cells 2, cB2) and IgD(-)CD27(+) memory B cells (cB3) in comparison with IgD(+)CD27(-) naive B cells (cB1). cB2 were found to be morphologically similar to cB3 with abundant cytoplasm, whereas cB3 expressed CD80, CD86, and CD95 on their surface more predominantly than cB2. A majority of cB2 expressed both IgD and IgM, and cB3 expressed IgA or IgG. Mature gamma1 and gamma2 transcripts were found in cB3, but at very low levels in cB2, and activation-induced cytidine deaminase (AID) mRNA expression was recognized only in cB3. The frequencies of somatic hypermutation in cB2 and cB3 were comparable levels studied by VH5. cB2 did not shift to cB3 in vitro by the stimuli such as via B-cell receptor or CD40. cB2 produced large amounts of IgM predominantly and promptly, which is in accordance with the known characteristics of memory B cells. Taken together, although cB2 are unclass-switched, cB2 have the functions of memory B cells and are not in the process of transition from naive to switched memory B cells, playing a crucial role in secondary immune response by producing high-affinity IgM in the early phase of infections.
机译：通过使用CD27作为记忆B细胞标记物，已经在各种疾病中测定了外周血中记忆B细胞的数量。然而，由免疫球蛋白（Ig）D表达分隔的两个记忆B细胞亚群之间在特征和功能方面的定义差异仍有待明确阐明。我们在这里分析了IgD（+）CD27（+）B细胞（循环B细胞2，cB2）和IgD（-）CD27（+）记忆B细胞（cB3）与IgD（+）CD27（-）幼稚B细胞相比（cB1）。发现cB2在形态上类似于具有丰富细胞质的cB3，而cB3在其表面上比cB2更主要表达CD80，CD86和CD95。大多数cB2表达IgD和IgM，而cB3表达IgA或IgG。在cB3中发现了成熟的gamma1和gamma2转录本，但在cB2中的含量很低，并且激活诱导的胞苷脱氨酶（AID）mRNA表达仅在cB3中被识别。 VB5研究了cB2和cB3中体细胞超突变的频率。在体外，cB2不会通过刺激（例如通过B细胞受体或CD40）转移到cB3。 cB2主要并迅速产生大量IgM，这与记忆B细胞的已知特征一致。综上所述，尽管cB2是非类别转换的，但cB2具有记忆B细胞的功能，并且不处于从幼稚到转换记忆B细胞的转变过程中，它通过在细胞中产生高亲和力IgM而在继发免疫反应中发挥关键作用。感染的早期阶段。

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