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首页> 外文期刊>Clinical immunology: The official journal of the Clinical Immunology Society >CD4(+) T cell clones producing both interferon-gamma and interleukin-10 predominate in bronchoalveolar lavages of active pulmonary tuberculosis patients.
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CD4(+) T cell clones producing both interferon-gamma and interleukin-10 predominate in bronchoalveolar lavages of active pulmonary tuberculosis patients.

机译：在活跃的肺结核患者的支气管肺泡灌洗中，产生干扰素-γ和白介素10的CD4（+）T细胞克隆占主导地位。

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The pattern of cytokine production in T cell clones derived from bronchoalveolar lavages (BAL) of active pulmonary tuberculosis (TB) patients was analyzed in clones obtained by limiting dilution procedures which expand with high efficiency either total T lymphocytes, independently of their antigen-recognition specificity, or Mycobacterium tuberculosis-specific T cells. BAL-derived clones, representative of CD4(+) cells from five patients with active TB, produced significantly higher amounts of IFN-gamma than BAL-derived CD4(+) clones from three inactive TB donors or four controls (with unrelated, noninfectious pathology). Average IL-4 and IL-10 production did not differ significantly in the three groups. Although these data suggest a predominant Th1 response to M. tuberculosis infection in the lungs, the majority of BAL-derived CD4(+) clones produced both IFN-gamma and IL-10 and the percentage of clones with this pattern of cytokine production was significantly higher in clones derived from BAL of active TB patients than from controls. Only rare clones derived from peripheral blood (PB)-derived CD45RO(+) CD4(+) T cells of both patients (nine cases) and controls (four cases) produced both IFN-gamma and IL-10; instead, the IL-10-producing clones derived from PB T cells most often also produced IL-4, displaying a typical Th2 phenotype. Higher average amounts of IFN-gamma and IL-10 were produced by BAL-derived CD8(+) clones of four active TB patients than of four controls, although the frequency of CD8(+) clones producing both IFN-gamma and IL-10 was lower than that of CD4(+) clones. The M. tuberculosis-specific BAL-derived T cell clones from three active TB patients were almost exclusively CD4(+) and produced consistently high levels of IFN-gamma often in association with IL-10, but very rarely with IL-4. Unlike the BAL-derived clones, the M. tuberculosis-specific clones derived from PB CD45RO(+) CD4(+) T cells of three different active TB patients and two healthy donors showed large individual variability in cytokine production as well as in the proportion of CD4(+), CD8(+), or TCR gamma/delta(+) clones. These results indicate the predominance of CD4(+) T cells producing both the proinflammatory cytokine IFN-gamma and the anti-inflammatory cytokine IL-10 in BAL of patients with active TB. Copyright 1999 Academic Press.
机译：在通过有限稀释程序获得的克隆中分析了活动性肺结核（TB）患者源自支气管肺泡灌洗液（BAL）的T细胞克隆中细胞因子的产生模式，该稀释程序可高效扩增全部T淋巴细胞，而不受其抗原识别特异性或结核分枝杆菌特异性T细胞。 BAL衍生的克隆代表了5名活动性结核病患者的CD4（+）细胞，与来自3个非活性TB供体或四个对照的BAL衍生CD4（+）克隆相比，产生的IFN-γ量明显更高）。三组的平均IL-4和IL-10产量无显着差异。尽管这些数据表明肺部结核分枝杆菌感染主要发生Th1反应，但大多数BAL衍生的CD4（+）克隆均产生IFN-γ和IL-10，且具有这种细胞因子产生模式的克隆百分比显着来自活动性结核病患者BAL的克隆中的克隆比对照组中的克隆更高。只有患者（9例）和对照（4例）的外周血（PB）衍生的CD45RO（+）CD4（+）T细胞衍生的罕见克隆才产生IFN-γ和IL-10。取而代之的是，从PB T细胞衍生的产生IL-10的克隆也经常产生IL-4，表现出典型的Th2表型。尽管4个活跃的TB患者的BAL来源的CD8（+）克隆产生的IFN-γ和IL-10的平均频率高于四个对照组，但CD8（+）克隆产生IFN-γ和IL-10的频率更高低于CD4（+）克隆。来自三名活动性结核病患者的结核分枝杆菌特异性BAL来源的T细胞克隆几乎完全是CD4（+），并经常与IL-10联合产生高水平的IFN-γ，但很少与IL-4结合。与BAL来源的克隆不同，结核分枝杆菌特异性克隆来自三个不同的活动性TB患者和两个健康捐献者的PB CD45RO（+）CD4（+）T细胞，在细胞因子产生和比例方面存在很大的个体差异CD4（+），CD8（+）或TCRγ/δ（+）克隆。这些结果表明在活动性结核病患者的BAL中，CD4（+）T细胞占主导地位，既产生促炎细胞因子IFN-γ，又产生抗炎细胞因子IL-10。版权所有1999，学术出版社。

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