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首页> 外文期刊>Clinical immunology: The official journal of the Clinical Immunology Society >Islet-cell antigen-reactive T cells show different expansion rates and Th1/Th2 differentiation in type 1 diabetic patients and healthy controls.
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Islet-cell antigen-reactive T cells show different expansion rates and Th1/Th2 differentiation in type 1 diabetic patients and healthy controls.

机译：胰岛细胞抗原反应性T细胞在1型糖尿病患者和健康对照组中显示出不同的扩增速率和Th1 / Th2分化。

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The low frequency of islet-cell antigen-reactive T cells in type 1 diabetes makes their direct measurement difficult. Commonly used in vitro expansion could alter in vivo frequencies and Th1/Th2 differentiation states. Using IFN-gamma/IL-4 double color ELISPOT, we tested longitudinally the reactivity of PBMC from HLA-matched diabetic patients and healthy controls to GAD65, IA-2, and proinsulin peptides ex vivo and after in vitro culture. The peptide-reactive T cells showed IFN-gamma bias in the patients' PBMC in the primary assay. During in vitro culture, both IFN-gamma- and IL-4-producing cells were induced in controls, suggesting that the precursor cells were uncommitted naive T cells in vivo. In contrast, in diabetic patients, the ex vivo IFN-gamma response was conserved during culture, suggesting their Th1 commitment. Using CFSE-dye-dilution, we demonstrate that naive T cells expand in vitro at a faster rate than memory cells, which might account for the differences in expansion rates between diabetic patients and controls.
机译：1型糖尿病中胰岛细胞抗原反应性T细胞的频率低，使其直接测量变得困难。常用的体外扩增可改变体内频率和Th1 / Th2分化状态。使用IFN-γ/ IL-4双色ELISPOT，我们纵向测试了HLA匹配的糖尿病患者和健康对照组的PBMC对离体和体外培养的GAD65，IA-2和胰岛素原肽的反应性。在主要试验中，与肽反应的T细胞在患者的PBMC中显示IFN-γ偏倚。在体外培养期间，在对照中均诱导了产生IFN-γ和IL-4的细胞，这表明该前体细胞是体内未定型的幼稚T细胞。相反，在糖尿病患者中，体外IFN-γ应答在培养过程中是保守的，表明他们的Th1承诺。使用CFSE染料稀释法，我们证明了天然T细胞的体外扩增速度要快于记忆细胞，这可能解释了糖尿病患者和对照组之间扩增速度的差异。

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