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首页> 外文期刊>Journal of Reproductive Immunology >Granulocyte-macrophage colony-stimulating factor (GM-CSF) targets myeloid leukocytes in the uterus during the post-mating inflammatory response in mice.
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Granulocyte-macrophage colony-stimulating factor (GM-CSF) targets myeloid leukocytes in the uterus during the post-mating inflammatory response in mice.

机译：在小鼠交配后的炎症反应过程中，粒细胞巨噬细胞集落刺激因子（GM-CSF）靶向子宫中的髓样白细胞。

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Factors in seminal plasma elicit a surge of GM-CSF expression in uterine epithelial cells after mating in mice. This study investigates the nature of the endometrial cell populations targeted by epithelial GM-CSF. In quantitative RT-PCR studies, expression of the alpha-subunit of the GM-CSF receptor (GM-CSF-R) parallelled GM-CSF expression, being maximal during the 48 h period after mating and declining thereafter. Expression of mRNA encoding beta-common chain (AIC2B) also increased after mating and remained high until the time of embryo implantation on day 4 of pregnancy. Cells expressing GM-CSF receptors were identified in sections of uterus on the day after mating using 125I-GM-CSF, and were located predominantly in the endometrial stroma subjacent to the luminal epithelium, co-localising with abundant populations of myeloid leukocytes. Cells expressing GM-CSF receptor were identified as macrophages, granulocytes and putative dendritic cells by flow cytometric analysis using lineage and receptor subunit specific antibodies. Recombinant GM-CSF injected into the uterine lumen of ovariectomised mice was found to elicit a dose-dependant accumulation of macrophages and granulocytes in the endometrium, in a pattern of distribution comparable to that seen in uteri after natural mating. Together, these data indicate a role for epithelial cell-derived GM-CSF in mediating the recruitment and potentially in modifying the behaviour of uterine leukocytes during the post-mating inflammatory response in mice.
机译：小鼠交配后，精浆中的因子引起子宫上皮细胞中GM-CSF表达激增。这项研究调查了上皮GM-CSF靶向的子宫内膜细胞群的性质。在定量RT-PCR研究中，GM-CSF受体（GM-CSF-R）的α亚基的表达与GM-CSF的表达平行，在交配后48小时内达到最大值，此后下降。交配后，编码β-公共链（AIC2B）的mRNA的表达也增加，并且一直保持高水平，直到怀孕第4天胚胎植入时为止。在交配后的第二天，使用125 I-GM-CSF在子宫切片中鉴定出表达GM-CSF受体的细胞，这些细胞主要位于子宫内膜基质内，靠近腔上皮，与大量髓样白细胞共定位。通过使用谱系和受体亚基特异性抗体的流式细胞术分析，将表达GM-CSF受体的细胞鉴定为巨噬细胞，粒细胞和推定的树突状细胞。发现将重组GM-CSF注射到去卵巢小鼠的子宫腔中会引起子宫内膜中巨噬细胞和粒细胞的剂量依赖性积累，其分布方式与自然交配后子宫中的分布相当。总之，这些数据表明源自上皮细胞的GM-CSF在介导小鼠交配后炎症反应期间介导募集并潜在地改变子宫白细胞的行为。

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