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首页> 外文期刊>Journal of Plantation Crops >High frequency Agrobacterium mediated genetic transformation in rubber tree via. vacuum infiltration
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High frequency Agrobacterium mediated genetic transformation in rubber tree via. vacuum infiltration

机译：高频农杆菌介导的橡胶树遗传转化。真空渗透

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In a tree species like Hevea brasiliensis, genetic transformation offers a viable approach for crop improvement within a short period. Hevea being highly recalcitrant to in vitro culture, an efficient transformation protocol is necessary for generating large number of transgenic plants with stable foreign gene expression. After several modifications in the initial transformation protocol, the transformation frequency was increased to 14 per cent using proliferated anther callus. Therefore, vacuum infiltration was attempted for enhancing the transformation frequency. In the present work the conditions for vacuum infiltration viz. vacuum pressure and period of infiltration were standardized. Vacuum infiltration at 30 psi pressure for 10 minutes was found to be ideal for getting high frequency transformation in H. brasiliensis. Irrespective of the gene constructs experimented, transformation frequency was significantly improved by adopting vacuum infiltration. Employing this technique, the transformation efficiency of MnSOD gene construct with FM V34S promoter could be enhanced from 14 to 50 per cent.
机译：在像巴西橡胶树这样的树种中，遗传转化为短期内的作物改良提供了可行的方法。橡胶树对体外培养具有很高的抵抗力，有效的转化方案对于产生大量具有稳定的外源基因表达的转基因植物是必需的。在对初始转化方案进行了几次修改之后，使用增殖的花药愈伤组织将转化频率提高到14％。因此，尝试了真空渗透以提高转化频率。在本工作中，真空渗透的条件也就是。真空压力和渗透时间标准化。发现在30 psi压力下进行10分钟的真空渗透对于在巴西布鲁氏菌中进行高频转化是理想的。不管实验的基因构建体是什么，通过采用真空渗透技术都可以显着提高转化频率。利用该技术，具有FM V34S启动子的MnSOD基因构建体的转化效率可以提高14％至50％。

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