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首页> 外文期刊>Clinical immunology: The official journal of the Clinical Immunology Society >Noncognate binding to histones of IgG from patients with idiopathic systemic lupus erythematosus.
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Noncognate binding to histones of IgG from patients with idiopathic systemic lupus erythematosus.

机译：非同源性与特发性系统性红斑狼疮患者的IgG组蛋白结合。

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The mechanism of attachment of circulating immune complexes (CIC) to glomerular basement membranes (GBM) has not yet been elucidated. Since it has been proposed that histone may be the ligand between GBM and DNA/anti-DNA CIC, we explored by ELISA and Western blots the nature of the interaction of IgG with histone on solid phase. Cognate binding of IgG anti-histone antibody was characteristically dependent on in its F(ab')(2) fragment and was inhibited by free histone. On the other hand, heat-aggregated IgG, a model for CIC, and IgG from most patients with idiopathic SLE had a characteristic noncognate binding behavior to histone: it was dependent on Fcgamma rather than on the F(ab')(2) fragment and was not effectively inhibited by free histones. Also, binding to histone of in vitro generated DNA/anti-DNA immune complexes was not dependent on DNA as a histone ligand, but on Fcgamma. Finally, there was good agreement between the binding of this IgG to histone and to C1q. We concluded that: (1) altered IgG and/or CIC bind to solid-phase-attached histone primarily through their Fcgamma and (2) CIC may mimic IgG antihistone antibodies in solid-phase immunoassays. Copyright 2000 Academic Press.
机译：循环免疫复合物（CIC）附着在肾小球基底膜（GBM）上的机制尚未阐明。由于已经提出组蛋白可能是GBM和DNA /抗DNA CIC之间的配体，因此我们通过ELISA和Western印迹探索了IgG与组蛋白在固相上相互作用的性质。 IgG抗组蛋白抗体的同源结合特征性地依赖于其F（ab'）（2）片段，并受到游离组蛋白的抑制。另一方面，来自大多数特发性SLE患者的热聚集IgG（一种CIC模型）和IgG具有与组蛋白的特征性非同源结合行为：它依赖于Fcγ而不是F（ab'）（2）片段并不受游离组蛋白的有效抑制。而且，与体外产生的DNA /抗DNA免疫复合物的组蛋白结合不依赖于DNA作为组蛋白配体，而是依赖于Fcγ。最后，在该IgG与组蛋白和C1q的结合之间有很好的一致性。我们得出的结论是：（1）改变的IgG和/或CIC主要通过其Fcγ与固相连接的组蛋白结合，并且（2）CIC在固相免疫测定中可以模拟IgG抗组蛋白抗体。版权所有2000学术出版社。

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