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首页> 外文期刊>Journal of Poultry Science >Analysis of Changes in the Expression of Transforming Growth Factor-beta s in the Utero-vaginal Junction of Hen Oviduct in Response to Sperm Concerning Their Significance in Sperm Survivability
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Analysis of Changes in the Expression of Transforming Growth Factor-beta s in the Utero-vaginal Junction of Hen Oviduct in Response to Sperm Concerning Their Significance in Sperm Survivability

机译：母猪输卵管子宫-阴道交界处转化生长因子β的表达变化对精子存活意义的分析

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摘要

The aim of present study was to determine whether the expression of transforming growth factor-beta s (TGF beta s) in the utero-vaginal junction (UVJ) of hen oviduct was changed by the stimulation with sperm. Localization of immunoreactive TGF beta 3 (irTGF beta 3) in the UVJ that contained sperm storage tubules (SST) were examined by immunocytochemistry with or without insemination. Changes in the mRNA expression of three types TGF beta isoforms (TGF beta 2, beta 3 and beta 4) in the vagina or UVJ cells co-cultured with fresh chicken sperm were observed by real-time PCR. The irTGF beta 3 were localized in the surface epithelia of UVJ of all the inseminated and non-inseminated birds. The immunoreaction products were not present in the SST cells of non-inseminated hens but were appeared in the SST at 1 d of insemination, and showed positive staining even after 10 or 20 d of insemination. The expression of three types TGF beta s were significantly increased in the cultured UVJ cells after stimulation by fresh sperm, however, the vaginal cells that were co-incubated with sperm did not show any alteration of their expression for all three types of TGF beta s. These results suggest that the interaction between sperm and SST cells may up-regulate TGF beta s expression in the SST which may play significance role in the sperm survival in SST.
机译：本研究的目的是确定是否通过精子刺激改变了输卵管子宫-阴道交界处的转化生长因子-β（TGF-β）的表达。通过免疫细胞化学（有或没有授精）检查了免疫反应性TGFβ3（irTGFβ3）在含有精子储存小管（SST）的UVJ中的定位。通过实时PCR观察到与新鲜鸡精液共培养的阴道或UVJ细胞中三种类型的TGFβ亚型（TGFβ2，β3和β4）的mRNA表达变化。 irTGF beta 3位于所有受精和未受精鸟类的UVJ表面上皮中。免疫反应产物在未受精母鸡的SST细胞中不存在，但在受精1 d时出现在SST中，甚至在受精10或20 d后仍显示阳性染色。新鲜精子刺激后，培养的UVJ细胞中三种类型的TGFβ的表达显着增加，但是，与精子共同孵育的阴道细胞对于这三种类型的TGFβ均未显示其表达的任何改变。这些结果表明，精子与SST细胞之间的相互作用可能会上调SST中TGFβ的表达，这可能在SST精子存活中起重要作用。

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