Cross-Species Utility of Microsatellite Markers in Trichostrongyloid Nematodes

Temperley Nicholas D.; Webster Lucy M. I.; Adam Aileen; Keller Lukas F.; Johnson Paul C. D.

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Cross-Species Utility of Microsatellite Markers in Trichostrongyloid Nematodes

机译：线粒体线虫中微卫星标记的跨物种效用

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The development of microsatellite markers for parasitic nematodes has been hampered by technical difficulties in isolation and PCR amplification. We have investigated the potential for circumventing these problems using microsatellites from 3 trichostrongyloid species on a panel of 7 species. Ten of the 22 PCR primer pairs tested amplified in species other than the target species, usually in closely related species, and 2 new variable loci were discovered in the sheep parasite Trichostrongylus vitrinus. This study provides evidence that cross-species testing of microsatellite primers can be an effective alternative to isolation de novo.

机译：分离线虫和PCR扩增的技术难题阻碍了用于寄生线虫的微卫星标记的发展。我们已经研究了使用来自7种物种的3个毛线虫类物种的微卫星来规避这些问题的潜力。在22个PCR引物对中，有10个在目标物种以外的物种中扩增，通常在密切相关的物种中扩增，并且在绵羊寄生虫玻璃藻Trichostrongylus vitrinus中发现了2个新的可变基因座。这项研究提供了证据，即微卫星引物的跨物种测试可以作为从头分离的有效替代方法。

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《Journal of Parasitology》 |2009年第2期|共3页
作者
Temperley Nicholas D.; Webster Lucy M. I.; Adam Aileen; Keller Lukas F.; Johnson Paul C. D.;
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正文语种 eng
中图分类寄生动物、寄生虫学;
关键词
Parasitology; Biochemistry and Molecular Biophysics;

机译：寄生虫学;生物化学与分子生物物理学;

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1. Cross-Species Utility of Microsatellite Markers in Trichostrongyloid Nematodes [J] . Temperley Nicholas D., Webster Lucy M. I., Adam Aileen, Journal of Parasitology . 2009,第2期

机译：线粒体线虫中微卫星标记的跨物种效用
2. Development of novel microsatellite markers for the Northern Goshawk (Accipiter gentilis) and their utility in cross-species amplification [J] . Haughey Christy L., Sage George K., DeGange Gabriel R., Avian Biology Research . 2016,第3期

机译：北部苍鹰（Accipiter gentilis）的新型微卫星标记的开发及其在跨物种扩增中的应用
3. Conserved microsatellite markers of high cross-species utility for flying, ground and tree squirrels [J] . Jumpa Salisa, Dawson Deborah A., Horsburgh Gavin J., Conservation Genetics Resources . 2015,第2期

机译：用于飞行，地面和树木松鼠的高跨物种实用性的保守微卫星标记
4. Application of microsatellite markers as marker assisted selection (MAS) in the F3 generation results crosses of black rice and white rice [C] . Kristamtini, Taryono, Panjisakti Basunanda, International Conference on Biological Science . 2020

机译：微卫星标记作为标记辅助选择（MAS）在F3代辅助选择的黑米和白米的交叉中的应用
5. Characterization of root-knot nematode resistance in cowpea and utilization of cross-species platforms in legume genomics [D] . Das, Sayan 2008

机译：cow豆根结线虫抗性的表征及豆科植物基因组学中跨物种平台的利用
6. Development of new genomic microsatellite markers from robusta coffee (Coffea canephora Pierre ex A. Froehner) showing broad cross-species transferability and utility in genetic studies [O] . Prasad Suresh Hendre, Regur Phanindranath, V Annapurna, 2008

机译：从罗布斯塔咖啡（Coffea canephora Pierre ex A. Froehner）开发新的基因组微卫星标记显示出广泛的跨物种转移能力和在遗传研究中的实用性
7. Conserved microsatellite markers of high cross-species utility for flying, ground and tree squirrels [O] . 2015

机译：用于飞行，地面和树木松鼠的高跨物种实用性的保守微卫星标记

Cross-Species Utility of Microsatellite Markers in Trichostrongyloid Nematodes

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