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首页> 外文期刊>Journal of Parasitology >Experimental Infection of the Mayan Cichlid Cichlasoma urophthalmus with the Oomycete Aphanomyces invadans
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Experimental Infection of the Mayan Cichlid Cichlasoma urophthalmus with the Oomycete Aphanomyces invadans

机译：卵菌卵圆孔菌感染玛雅丽鱼科鱼眼鱼的实验性感染

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摘要

This study aimed to determine the susceptibility of the Mayan cichlid Cichlasoma urophthalmus to infection with the fungus Aphanomyces invadans (also known as epizootic ulcerative syndrome [EUS]). A total of 27 C. urophthalmus were exposed to the original A. Invadans 2006/86/EC strain by intramuscularly injecting the fish with 25,000 zoospores/ml or exposing the fish to a suspension of 25,000 zoospores/ml in 6-L aquaria for 30 days. To assess the infectious capacity of A. invadans, 3 golden barbs (Puntius semifasciolatus) were infected intramuscularly with 200,000 zoospores/ml. A second experiment using 100 C. urophthalmus was performed for 60 days with 50 fish in each treatment group. A polymerase chain reaction (PCR)-based diagnostic method was used; muscle and gills were the target tissues. In the first experiment, none of the exposed C. urophthalmus developed skin lesions related to A. invadans infection. However, PCR analysis revealed that infection had occurred. For the intramuscular treatment, there were significant differences between the controls and the muscle samples (Fisher's exact test; P 0.05) but not between the controls and the gill samples (P > 0.05). All golden barbs became infected, as indicated by PCR, and developed skin lesions typical of A. invadans infection. We concluded that C. urophthalmus was infected with A. invadans but was an asymptomatic carrier because skin lesions did not develop. In the second experiment, all fish were negative, suggesting that the fish had cleared the infection by the end of the experiment.
机译：这项研究旨在确定玛雅丽鱼科鱼ichichlasoma urophthalmus对真菌Aphanomyces invadans（也称为流行性溃疡综合症[EUS]）感染的敏感性。通过肌肉内向鱼注射25,000个游动孢子/ ml或将鱼暴露于25,000个游动孢子/ ml在6升水族箱中的悬浮液中30次，使总共27个尿道弯曲杆菌暴露于原始的A. Invadans 2006/86 / EC菌株。天。为了评估A. invadans的感染能力，将3个金倒钩（Puntius semifasciolatus）肌肉内感染了200,000个游动孢子/ ml。在每个处理组中对50只鱼进行了使用100例尿道弯曲杆菌的第二次实验，为期60天。使用基于聚合酶链反应（PCR）的诊断方法;肌肉和g是目标组织。在第一个实验中，没有一个暴露的尿道隐球菌发生与侵染曲霉有关的皮肤损伤。但是，PCR分析表明已发生感染。对于肌肉内治疗，对照组和肌肉样本之间存在显着差异（Fisher精确检验; P <0.05），而对照组和腮样本之间无显着差异（P> 0.05）。如PCR所示，所有的金色倒钩都被感染，并发展出典型的侵袭阿瓦德氏菌感染的皮肤损伤。我们得出的结论是，尿眼衣原体感染了侵华曲霉，但由于皮肤病变未发生，因此是无症状的携带者。在第二个实验中，所有鱼均为阴性，表明该鱼在实验结束时已清除感染。

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