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首页> 外文期刊>Journal of the Korean Society for Applied Biological Chemistry >Characterization of Artificially Induced Cadmium-tolerant Yeast Mutants
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Characterization of Artificially Induced Cadmium-tolerant Yeast Mutants

机译：人工诱导的耐镉酵母突变体的表征

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Bioremediation of heavy metals by using microorganisms is an effective strategy in regions that have low and wide-ranging metal concentrations and in situations where physical and chemical techniques are not suitable. Because of their higher capacity to remove a wide range of metals by biosorption, yeasts are useful for the bioremediation of heavy metals. In this study, identification of yeast mutants (CdRs) was focused, which have strong resistance to cadmium (Cd), a representative heavy metal. Yeast cells were sequentially adapted to gradually increasing the Cd concentration up to 30 mM. The resultant mutant, CdR30 cells survived a final Cd concentration of 30 mM, while the control cells failed to survive at 0.5 mM in 7 d. It was analyzed whether the increased Cd tolerance of the mutants was associated with sensitivity toward other metals. Compared to control cells, CdR20 cells showed increased tolerance to Cu, decreased tolerance to Ni, and comparable tolerance to Zn. However, these tolerances were not reproducible, because CdRs isolated in a second round of induction showed different metal sensitivities. The increase in Ni sensitivity in CdR20 cells was overcome by performing a second adaptation to Ni stress. Thus, CdR20 cells that were tolerant to both Cd and Ni were generated. These data presented in this study may be useful for the application of microorganisms to the bioremediation of heavy metals
机译：使用微生物对重金属进行生物修复是在金属浓度低而范围广泛的地区以及在物理和化学技术不合适的情况下的有效策略。由于酵母具有更高的通过生物吸附去除多种金属的能力，因此可用于生物修复重金属。在这项研究中，重点是对具有代表性的重金属镉（Cd）具有较强抗性的酵母突变体（CdRs）的鉴定。酵母细胞顺序适应以逐渐将Cd浓度增加至30 mM。最终的突变CdR30细胞在30 dM的最终Cd浓度下存活，而对照细胞在7 d内未能以0.5 mM存活。分析了突变体对Cd的耐受性增加是否与对其他金属的敏感性相关。与对照细胞相比，CdR20细胞显示出对Cu的耐受性增加，对Ni的耐受性下降，以及对Zn的耐受性。但是，由于在第二轮诱导中分离出的CdR表现出不同的金属敏感性，因此这些耐受性无法再现。通过对Ni胁迫进行第二次适应，克服了CdR20细胞中Ni敏感性的增加。因此，产生了对Cd和Ni均耐受的CdR20细胞。这项研究中提供的这些数据可能对微生物在重金属的生物修复中的应用很有用。

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