Transient gene expression in Japanese lawngrass (Zoysia japonica Steud.) protoplasts.

Inokuma C; Sugiura K; Okawara R; Cho C

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Transient gene expression in Japanese lawngrass (Zoysia japonica Steud.) protoplasts.

机译：日本草坪草（Zoysia japonica Steud。）原生质体中的瞬时基因表达。

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Conditions for maximizing transient expression and activity of 4 promoter/intron constructs in protoplasts of Z. japonica were investigated. Transient expression of the GUS [uidA] reporter gene showed that polyethylene glycol (PEG)-mediated plasmiduptake largely depended on PEG concentration, plasmid quantity and heat shock treatment of protoplasts before the addition of PEG. In the transient assay using 4 constructs, rice actin 1 promoter showed the highest level of transgene expression, suggesting that it is efficient for use in the genetic transformation of Japanese lawngrass.

机译：研究了最大化Z. japonica原生质体中4个启动子/内含子构建体的瞬时表达和活性的条件。 GUS [uidA]报告基因的瞬时表达表明，聚乙二醇（PEG）介导的质粒摄取在很大程度上取决于PEG浓度，质粒数量和添加PEG之前原生质体的热激处理。在使用4种构建体进行的瞬时分析中，水稻肌动蛋白1启动子显示出最高水平的转基因表达，表明它可有效用于日本草的遗传转化。

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《Journal of Turfgrass Management》 |1997年第3期|共10页
作者
Inokuma C; Sugiura K; Okawara R; Cho C;
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正文语种 eng
中图分类园艺;
关键词
polyethylene glycol; protoplasts; gene expression; protoplast culture; promoters; reporter genes; lawns and turf; ornamental plants; biotechnology; Zoysia japonica;

机译：聚乙二醇;原生质体;基因表达;原生质体培养;启动子;报告基因;草坪和草皮;观赏植物;生物技术;结缕草;

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1. Transient gene expression in Japanese lawngrass (Zoysia japonica Steud.) protoplasts. [J] . Inokuma C, Sugiura K, Okawara R, Journal of Turfgrass Management . 1997,第3期

机译：日本草坪草（Zoysia japonica Steud。）原生质体中的瞬时基因表达。
2. Corrigendum: De novo assembly of the Japanese lawngrass ( Zoysia japonica Steud.) root transcriptome and identification of candidate unigenes related to early responses under salt stress [J] . Qi Xie, Jun Niu, Xilin Xu, Frontiers in Plant Science . 2015,第1期

机译：更正：日本草（根结实）根转录组的从头组装和与盐胁迫下早期响应相关的候选单基因的鉴定
3. De novo assembly of the Japanese lawngrass ( Zoysia japonica Steud.) root transcriptome and identification of candidate unigenes related to early responses under salt stress [J] . Qi Xie, Jun Niu, Xilin Xu, Frontiers in Plant Science . 2015,第1期

机译：盐草根系的 De novo 组装和盐胁迫下与早期响应相关的候选单基因的鉴定
4. Effects of Sward Height at the End of Each Rotational Grazing Period on the Number of Ears in Grazed Japanese Lawngrass (Zoysia japonica Steud.) Pastures [C] . Makoto Kaneko, Yuzo Kurokawa, Qing Hai, New paradigm for diversity of forage production in the east Asian region . 2009

机译：旋转放牧结束时草皮高度对放牧日本草（草地结缕草）牧场的穗数的影响
5. Fungal pathogens associated with seasonal dormancy of Zoysia japonica: The behavior and interaction of Gaeumannomyces incrustans and Rhizoctonia solani, coinhabitants [D] . Curry, Elizabeth Sue 2009

机译：与日本结缕草的季节性休眠有关的真菌病原体：拟南牛（Gaeumannomyces incrustans）和茄红假单胞菌（Rhizoctonia solani）的行为和相互作用
6. De novo assembly of the Japanese lawngrass (Zoysia japonica Steud.) root transcriptome and identification of candidate unigenes related to early responses under salt stress [O] . Qi Xie, Jun Niu, Xilin Xu, 2015

机译：日本草（Zoysia japonica Steud。）根转录组的从头组装和鉴定与盐胁迫下早期响应相关的候选单基因
7. Corrigendum: De novo assembly of the Japanese lawngrass (Zoysia japonica Steud.) root transcriptome and identification of candidate unigenes related to early responses under salt stress [O] . Qi eXie 2015

机译：勘误表：日本草坪草（Zoysia japonica steud。）根瘤转录组的初始组装和与盐胁迫下早期反应相关的候选unigenes的鉴定
8. One Generation Reproductive Toxicity Test in Japanese quail (Coturnix japonica) Exposed Orally to 3-nitro-1,2,4-triazol 5 one (NTO), February-August 2015. [R] . Jackovitz, A. M. 2017

机译：日本鹌鹑（Coturnix japonica）的一代生殖毒性试验口服暴露于3-硝基-1,2,4-三唑-5（NTO），2015年2月至8月。

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Transient gene expression in Japanese lawngrass (Zoysia japonica Steud.) protoplasts.

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