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首页> 外文期刊>Journal of tissue engineering and regenerative medicine >Preparation and functional evaluation of cell aggregates incorporating gelatin microspheres with different degradabilities.
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Preparation and functional evaluation of cell aggregates incorporating gelatin microspheres with different degradabilities.

机译：掺入具有不同降解性的明胶微球的细胞聚集体的制备和功能评估。

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The objective of this study was to investigate the viability and biological functions of cells in their aggregates incorporating gelatin microspheres with different degradabilities. After being prepared by a water-in-oil emulsion procedure, the gelatin microspheres were dehydrothermally crosslinked at 140°C for various time periods. In vitro degradation tests showed that the gelatin microspheres were slowly degraded slowly with an increase in the crosslinking time. When MC3T3-E1 cells were cultured with the gelatin hydrogel microspheres in the round U-bottom wells of 96-well microplates which had been coated with poly(vinyl alcohol), cell aggregates with homogeneously distributed gelatin microspheres were formed. A large amount of slowly degraded gelatin microspheres remained in the cell aggregates for long time periods, while a higher proliferation of MC3T3-E1 cells was observed. When evaluated as a measure of aerobic glycolysis, the ratio of l-lactic acid production:glucose consumption of MC3T3-E1 cells was lower for MC3T3-E1 cells in the cell aggregates incorporating slowly degraded gelatin microspheres than for aggregates incorporating rapidly degraded ones. The alkaline phosphatase activity and calcium content of MC3T3-E1 cells were higher for cell aggregates incorporating slowly degraded gelatin microspheres. It is possible that the incorporation of gelatin hydrogel microspheres with slow degradability enabled the permeation of oxygen and nutrients into the cell aggregates for longer time periods, resulting in better culture conditions for the survival, proliferation and differentiation of the cells. Copyright ? 2012 John Wiley & Sons, Ltd.
机译：这项研究的目的是研究具有不同降解性的明胶微球的聚集体中细胞的活力和生物学功能。通过油包水乳液法制备后，将明胶微球在140℃下进行不同时间的脱氢热交联。体外降解试验表明，随着交联时间的增加，明胶微球缓慢降解。当用明胶水凝胶微球在涂有聚乙烯醇的96孔微孔板的圆形U形底部孔中培养MC3T3-E1细胞时，形成具有均匀分布的明胶微球的细胞聚集体。大量缓慢降解的明胶微球长时间保留在细胞聚集体中，同时观察到MC3T3-E1细胞具有更高的增殖能力。当评估为有氧糖酵解的量度时，MC3T3-E1细胞在掺入缓慢降解明胶微球的细胞聚集物中的L-乳酸产量与葡萄糖消耗的比率要比掺入快速降解的明胶微球的细胞聚集物中的MC3T3-E1细胞低。对于掺入缓慢降解的明胶微球的细胞聚集体，MC3T3-E1细胞的碱性磷酸酶活性和钙含量较高。缓慢降解的明胶水凝胶微球的掺入可能使氧气和营养物在更长的时间内渗透到细胞聚集体中，从而为细胞的存活，增殖和分化提供了更好的培养条件。版权？ 2012年John Wiley＆Sons，Ltd.

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