A rapid assay for detecting antibody against Bluetongue virus with a latex agglutination test using recombinant VP7 antigen.

Yang JunXing; Hua QunYi; Chen HuanChun; Lv JianQiang; Chen Bing; Ruan ZhouXi

首页> 外文期刊>Journal of Veterinary Diagnostic Investigation >A rapid assay for detecting antibody against Bluetongue virus with a latex agglutination test using recombinant VP7 antigen.

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A rapid assay for detecting antibody against Bluetongue virus with a latex agglutination test using recombinant VP7 antigen.

机译：使用重组VP7抗原的乳胶凝集试验，快速检测抗蓝舌病毒的抗体。

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摘要

A latex agglutination test (LAT) for detecting antibody against Bluetongue virus (BTV) in ruminants was developed using latex beads coupled with recombinant VP7 protein. Compared with competitive enzyme-linked immunosorbent assay (ELISA), the specificity and sensitivity of the LAT were 99.0% and 93.0%, respectively. There was excellent agreement between the results obtained by competitive ELISA and the LAT (kappa=0.930). Because it is rapid and easy to use, the LAT could be used for BTV antibody detection, especially for screening many serum samples.

机译：使用乳胶珠和重组VP7蛋白，开发了一种乳胶凝集试验（LAT），用于检测反刍动物中的蓝舌病毒（BTV）抗体。与竞争性酶联免疫吸附试验（ELISA）相比，LAT的特异性和敏感性分别为99.0％和93.0％。通过竞争性ELISA获得的结果与LAT之间有极好的一致性（kappa = 0.930）。由于LAT快速且易于使用，因此可用于BTV抗体检测，尤其是用于筛查许多血清样品。

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来源
《Journal of Veterinary Diagnostic Investigation》 |2010年第2期|共3页
作者
Yang JunXing; Hua QunYi; Chen HuanChun; Lv JianQiang; Chen Bing; Ruan ZhouXi;
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正文语种 eng
中图分类动物医学（兽医学）;
关键词
agglutination tests; antibodies; antibody testing; antigens; assays; diagnosis; ELISA; latex; latex agglutination test; rapid methods; screening; techniques; viral diseases; viruses; bluetongue virus; ruminants;

机译：凝集试验;抗体;抗体检测;抗原;化验;诊断;ELISA;乳胶;乳胶凝集试验;快速方法;筛选;技术;病毒疾病;病毒;蓝舌病毒;反刍动物;

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专利

1. A rapid assay for detecting antibody against Bluetongue virus with a latex agglutination test using recombinant VP7 antigen. [J] . Yang JunXing, Hua QunYi, Chen HuanChun, Journal of Veterinary Diagnostic Investigation . 2010,第2期

机译：使用重组VP7抗原的乳胶凝集试验，快速检测抗蓝舌病毒的抗体。
2. Comparison of the latex agglutination test with the hemagglutination inhibition test, enzyme-linked immunosorbent assay, and neutralization test for detection of antibodies to rubella virus. [J] . J M Meegan, B K Evans, D M Horstmann Journal of Clinical Microbiology . 1982,第4期

机译：乳胶凝集试验与血凝抑制试验，酶联免疫吸附试验和中和试验的比较，以检测风疹病毒抗体。
3. Development and assessment of a latex agglutination test based on recombinant MSP5 to detect antibodies against Anaplasma marginale in cattle [J] . Carlos A.n. Ramos, Carlos E.s. Vidal, Elaine S.p. Melo, Brazilian Journal of Microbiology . 2014,第1期

机译：基于重组MSP5的乳胶凝集试验的开发和评估，以检测牛抗浆膜炎的抗体
4. COMPARISON OF A DEVELOPMENTAL ELISA FOR ANTI-Erysipelothrix rhusiopathiae ANTIBODIES WITH A PASSIVE AGGLUTINATION ASSAY USING LATEX BEADS COATED WITH A PURIFIED 64 kDa E. rhusiopathiae SPECIFIC PROTEI [C] . John C. Jones, Bobby L. Middlebrooks, Rhonda A. Patterson Annual Conference of the International Association for Aquatic Animal Medicine . 2001

机译：使用胶乳珠子涂覆纯化的64kDae.Rousiopathiae特异性Protei的胶乳珠抗肠杆菌抗体的发育ELISA对抗炎症抗体的抗体抗体的比较
5. Purification of immunoglobulins, their bonding onto latex particles and the development of latex agglutination test for mouse immunoglobulin-G for monoclonal antibody assessment. [D] . Sompuram, Seshi Reddy. 1987

机译：免疫球蛋白的纯化，它们与乳胶颗粒的键合以及用于小鼠免疫球蛋白-G的乳胶凝集试验的发展，用于单克隆抗体评估。
6. Detection of antibodies to human immunodeficiency virus by latex agglutination with recombinant antigen. [O] . C H Riggin, G A Beltz, C H Hung, 1987

机译：通过重组抗原的乳胶凝集检测人免疫缺陷病毒的抗体。
7. Detection of rotavirus in faecal specimens with a monoclonal antibody enzyme-linked immunosorbent assay: Comparison with polyclonal antibody enzyme immuno-assays and a latex agglutination test [O] . M Sneyers, C Thiriart, C Buryns, 1989

机译：用单克隆抗体酶联免疫吸附试验检测粪便样品中的轮状病毒：与多克隆抗体酶免疫测定和胶乳凝集试验的比较
8. Detection of Rotavirus with a New Polyclonal Antibody Enzyme Immunoassay (Rotazyme 2) and a Commercial Latex Agglutination Text (Rotalex): Comparison with a Monoclonal Antibody Enzyme Immunoassay [R] . Doern, G. V. , Herrmann, J. E. , Henderson, P. , 1986

机译：用新的多克隆抗体酶免疫分析（Rotazyme 2）和商业乳胶凝集文本（Rotalex）检测轮状病毒：与单克隆抗体酶免疫分析比较

A rapid assay for detecting antibody against Bluetongue virus with a latex agglutination test using recombinant VP7 antigen.

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