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首页> 外文期刊>Journal of Virological Methods >Evaluation of an enzyme-linked immunosorbent assay for detection of antibodies to Junin virus in rodents
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Evaluation of an enzyme-linked immunosorbent assay for detection of antibodies to Junin virus in rodents

机译：评估小鼠啮齿类动物抗胡宁病毒抗体的酶联免疫吸附法的评估

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摘要

Junin virus is the etiological agent of Argentine hemorrhagic fever. a serious rodent-borne disease. An enzyme-linked immunosorbent assay (ELISA) to detect Junin virus IgG antibodies in rodents was evaluated using sera from 27 Calomys masculinus and five Calomys laucha, inoculated experimentally with a live attenuated strain of this arenavirus. The test performance was compared against an indirect immunofluorescence assay (IFA). The ELISA had a sensitivity and specificity of 100% and a reproducibility of 87.9% for samples with titers above the selected cut-off value. IFA had lower sensitivity (53%) with the same specificity. The ELISA results were similar, whether carried out on whole blood or serum samples, thus eliminating the need for serum separation, A high correlation (K = 0.86) between ELISA and IFA results was obtained from 1011 wild sigmodontine and marine rodents collected within and outside of the Argentine hemorrhagic fever endemic area. These results indicate that Junin virus IgG ELISA is the most suitable assay for detection of Junin virus antibodies in rodent samples. (C) 2002 Elsevier Science B.V. All rights reserved. [References: 32]
机译：胡宁病毒是阿根廷出血热的病原体。严重的啮齿动物传播疾病。酶联免疫吸附试验（ELISA）用于检测啮齿类动物中的Junin病毒IgG抗体，方法是使用27种Masomysus和5种laucha laucha血清进行评估，并用减毒活病毒减毒株进行实验接种。将测试性能与间接免疫荧光测定（IFA）进行了比较。对于滴定度高于所选截断值的样品，ELISA的灵敏度和特异性为100％，重现性为87.9％。 IFA具有相同的特异性，灵敏度较低（53％）。无论是在全血还是血清样品上进行的ELISA结果都是相似的，从而消除了血清分离的需要。从1011只野生西格达汀和海生啮齿动物体内外采集的ELISA与IFA结果之间具有高度相关性（K = 0.86）的阿根廷出血热流行地区。这些结果表明，Junin病毒IgG ELISA是最适合检测啮齿动物样品中Junin病毒抗体的检测方法。 （C）2002 Elsevier Science B.V.保留所有权利。 [参考：32]

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