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首页> 外文期刊>Journal of cellular biochemistry. >Mutual enhancement of differentiation of osteoblasts and osteocytes occurs through direct cell-cell contact
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Mutual enhancement of differentiation of osteoblasts and osteocytes occurs through direct cell-cell contact

机译：通过直接的细胞接触，相互促进成骨细胞和骨细胞分化

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There is increasing evidence that osteocytes regulate multiple aspects of bone remodeling through bi-directional communication with osteoblasts. This is potentially mediated through cell-cell contact via osteocytic dendritic processes, through the activity of secreted factors, or both. To test whether cell-cell contact affects gene expression patterns in osteoblasts and osteocytes, we used a co-culture system where calvarial osteoblasts and IDG-SW3 osteocytes were allowed to touch through a porous membrane, while still being physically separated to allow for phenotypic characterization. Osteoblast/osteocyte cell-contact resulted in up-regulation of osteoblast differentiation genes in the osteoblasts, when compared to wells where no cell contact was allowed. Examination of osteocyte gene expression when in direct contact with osteoblasts also revealed increased expression of osteocyte-specific genes. These data suggest that physical contact mutually enhances both the osteoblastic and osteocytic character of each respective cell type. Interestingly, Gja1 (a gap junction protein) was increased in the osteoblasts only when in direct contact with the osteocytes, suggesting that Gja1 may mediate some of the effects of direct cell contact. To test this hypothesis, we treated the direct contact system with the gap junction inhibitor 18-alpha-glycyrrhetinic acid and found that Bglap expression was significantly inhibited. This suggests that osteocytes may regulate late osteoblast differentiation at least in part through Gja1. Identification of the specific factors involved in the enhancement of differentiation of both osteoblasts and osteocytes when in direct contact will uncover new biology concerning how these bone cells communicate.
机译：越来越多的证据表明，骨细胞通过与成骨细胞的双向通讯来调节骨骼重塑的多个方面。这可能是通过细胞与细胞的接触，经由骨细胞的树突状过程，通过分泌因子的活性或两者而介导的。为了测试细胞之间的接触是否会影响成骨细胞和成骨细胞中的基因表达模式，我们使用了共培养系统，其中颅盖成骨细胞和IDG-SW3骨细胞可以通过多孔膜接触，同时仍可物理分离以进行表型鉴定。与不允许细胞接触的孔相比，成骨细胞/骨细胞的细胞接触导致成骨细胞中成骨细胞分化基因的上调。与成骨细胞直接接触时对骨细胞基因表达的检查还显示出骨细胞特异性基因的表达增加。这些数据表明，身体接触可以相互增强每种细胞类型的成骨细胞和骨细胞特性。有趣的是，仅当与成骨细胞直接接触时，成骨细胞中的Gja1（间隙连接蛋白）才会增加，这表明Gja1可能介导了直接细胞接触的某些作用。为了验证该假设，我们用间隙连接抑制剂18-α-甘草次酸处理了直接接触系统，发现Bglap表达被显着抑制。这表明骨细胞可能至少部分通过Gja1调节晚期成骨细胞的分化。直接接触成骨细胞和骨细胞分化增强的特定因素的鉴定将揭示有关这些骨细胞如何交流的新生物学。

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