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首页> 外文期刊>Journal of cellular biochemistry. >-Solanine Isolated From Solanum Tuberosum L. cv Jayoung Abrogates LPS-Induced Inflammatory Responses Via NF-B Inactivation in RAW 264.7 Macrophages and Endotoxin-Induced Shock Model in Mice
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-Solanine Isolated From Solanum Tuberosum L. cv Jayoung Abrogates LPS-Induced Inflammatory Responses Via NF-B Inactivation in RAW 264.7 Macrophages and Endotoxin-Induced Shock Model in Mice

机译：茄子茄中分离的茄碱通过RAW 264.7巨噬细胞中NF-B失活和内毒素诱导的小鼠休克模型废除LPS诱导的炎症反应

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摘要

-Solanine, a trisaccharide glycoalkaloid, has been reported to possess anti-cancer effects. In this study, we investigated the anti-inflammatory effects of -solanine isolated from Jayoung a dark purple-fleshed potato by examining its in vitro inhibitory effects on inducible nitric-oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and pro-inflammatory cytokines in LPS-induced RAW 264.7 macrophages and its in vivo effects on LPS-induced septic shock in a mouse model. -Solanine suppressed the expression of iNOS and COX-2 both at protein and mRNA levels and consequently inhibited nitric oxide (NO) and prostaglandin E-2 (PGE(2)) production in LPS-induced RAW 264.7 macrophages. -Solanine also reduced the production and mRNA expression of interleukin-6 (IL-6), tumor necrosis factor- (TNF-), and interleukin-1 (IL-1) induced by LPS. Furthermore, molecular mechanism studies indicated that -solanine inhibited LPS-induced activation of nuclear factor-B (NF-B) by reducing nuclear translocation of p65, degradation of inhibitory B (IB), and phosphorylation of IB kinase/ (IKK/). In an in vivo experiment of LPS-induced endotoxemia, treatment with -solanine suppressed mRNA expressions of iNOS, COX-2, IL-6, TNF-, and IL-1, and the activation of NF-B in liver. Importantly, -solanine increased the survival rate of mice in LPS-induced endotoxemia and polymicrobial sepsis models. Taken together, our data suggest that the -solanine may be a promising therapeutic against inflammatory diseases by inhibiting the NF-B signaling pathway. J. Cell. Biochem. 117: 2327-2339, 2016. (c) 2016 Wiley Periodicals, Inc.
机译：-茄碱，一种三糖类生物碱，据报道具有抗癌作用。在这项研究中，我们研究了从Jayoung分离出的深紫色肉马铃薯中获得的-茄碱的抗炎作用，方法是研究其对诱导型一氧化氮合酶（iNOS），环氧合酶2（COX-2）和LPS诱导的RAW 264.7巨噬细胞中的促炎性细胞因子及其在小鼠模型中对LPS诱导的败血性休克的体内作用。 -茄碱抑制iNOS和COX-2在蛋白质和mRNA水平上的表达，并因此抑制LPS诱导的RAW 264.7巨噬细胞中一氧化氮（NO）和前列腺素E-2（PGE（2））的产生。 -茄碱还降低了LPS诱导的白细胞介素6（IL-6），肿瘤坏死因子-（TNF-）和白细胞介素-1（IL-1）的产生和mRNA表达。此外，分子机制研究表明，-茄碱可通过减少p65的核易位，抑制性B的降解（IB）和IB激酶/（IKK /）的磷酸化来抑制LPS诱导的核因子B（NF-B）活化。在LPS诱导的内毒素血症的体内实验中，用-茄碱处理可抑制iNOS，COX-2，IL-6，TNF-和IL-1的mRNA表达以及肝中NF-B的活化。重要的是，β-茄碱在LPS诱导的内毒素血症和微生物败血症模型中提高了小鼠的存活率。两者合计，我们的数据表明，通过抑制NF-B信号通路，-茄碱可能是抗炎性疾病的有前途的治疗方法。 J.细胞。生化。 117：2327-2339，2016。（c）2016 Wiley Periodicals，Inc.

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