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首页> 外文期刊>Journal of cellular biochemistry. >BHBA Influences Bovine Hepatic Lipid Metabolism via AMPK Signaling Pathway
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BHBA Influences Bovine Hepatic Lipid Metabolism via AMPK Signaling Pathway

机译：BHBA通过AMPK信号通路影响牛肝脂质代谢

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摘要

beta-hydroxybutyric acid (BHBA), an important metabolite in beta-oxidation, is involved in the development of ketosis in dairy cows. It is known that AMP-activated protein kinase (AMPK) signaling pathway plays an important role in the regulation of lipid metabolism in hepatocytes. In the present study, bovine hepatocytes were treated with BHBA at variable concontrations and Compound C (Cpd C, an AMPK inhibitor) to investigate the effects of BHBA on the AMPK signaling pathway. The results showed that when the concentration of BHBA reached 1.2 mM, the AMPK signaling pathway was activated and the expression of sterol regulatory element binding protein-1c (SREBP-1c) as well as its target genes were significantly decreased. And these decreases were blocked by Cpd C. The binding activity and nucleus translocation of SREBP-1c showed a similar trend. The expression of peroxisome proliferator activated receptor-alpha (PPAR alpha), carbohydrates response element binding protein (ChREBP) and their target genes were significantly increased while they were negatively suppressed by the Cpd C. The content of triglyceride (TG) had no obviously change in the BHBA and Cpd C-treated groups. These results indicate that BHBA can activate AMPK signaling pathway and regulate lipid synthesis and lipid oxidation genes of AMPK but showed no effect on TG in bovine hepatocytes. (C) 2015 Wiley Periodicals, Inc.
机译：β-羟基丁酸（BHBA）是β-氧化中的重要代谢产物，参与了奶牛酮症的发展。已知AMP激活的蛋白激酶（AMPK）信号通路在肝细胞脂质代谢的调节中起着重要作用。在本研究中，牛肝细胞在不同的条件下用BHBA和化合物C（AMPK抑制剂Cpd C）处理，以研究BHBA对AMPK信号通路的影响。结果表明，当BHBA浓度达到1.2 mM时，AMPK信号通路被激活，固醇调节元件结合蛋白1c（SREBP-1c）及其靶基因的表达明显降低。这些减少被Cpd C阻止。SREBP-1c的结合活性和核易位显示出相似的趋势。过氧化物酶体增殖物激活受体α（PPAR alpha），碳水化合物反应元件结合蛋白（ChREBP）及其靶基因的表达显着增加，而被Cpd C抑制。甘油三酸酯（TG）的含量无明显变化在BHBA和Cpd C处理组中。这些结果表明，BHBA可以激活AMPK信号通路并调节AMPK的脂质合成和脂质氧化基因，但对牛肝细胞的TG没有影响。 （C）2015威利期刊公司

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