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首页> 外文期刊>Journal of Surgical Research: Clinical and Laboratory Investigation >Detection of telomerase activity in gastric lavage fluid: a novel method to detect gastric cancer.
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Detection of telomerase activity in gastric lavage fluid: a novel method to detect gastric cancer.

机译：洗胃液中端粒酶活性的检测：一种检测胃癌的新方法。

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摘要

BACKGROUND: Telomerase is a ribonucleoprotein polymerase that is essential for cell immortality. Recent studies have demonstrated that a high percentage of gastric cancer tissue expressed telomerase. This study describes the presence of telomerase activity in gastric lavage fluid in patients with gastric cancer. METHODS: Gastric lavage fluid was collected during esophageogastroduodenoscopy in 70 patients: 25 with gastric cancer, 25 with peptic ulcer disease, and 20 with normal stomach. The fluid and biopsy samples were analyzed for telomerase activity by a polymerase chain reaction-based telomerase repeat amplification protocol. The findings were related to the histological results. RESULTS: Telomerase activity was present in 24 of the 25 (96%) gastric cancer tissue and in 7 of the 25 tissue specimens from peptic ulcer or gastritis. In the gastric lavage fluid, telomerase was detected in 20 patients (80%) with gastric cancer, 7 patients (28%) with peptic ulcer, and none in normal subjects (P < 0.001). The sensitivity, specificity, positive predictive value, and negative predictive value of gastric fluid telomerase expression in gastric cancer patients was 80%, 84%, 74%, and 88%, respectively. CONCLUSIONS: The presence of telomerase activity is present in gastric lavage fluid of patients with gastric cancer as compared to those without, may represent a novel method for diagnosis of gastric cancer.
机译：背景：端粒酶是一种核糖核酸蛋白聚合酶，对细胞永生至关重要。最近的研究表明，胃癌组织中有很高的百分比表达端粒酶。这项研究描述了胃癌患者洗胃液中端粒酶活性的存在。方法：在食管胃十二指肠镜检查期间收集了70例胃灌洗液：25例胃癌，25例消化性溃疡病和20例正常胃。通过基于聚合酶链反应的端粒酶重复扩增方案分析了液体和活检样品的端粒酶活性。这些发现与组织学结果有关。结果：25种胃癌组织中有24种（96％）和25种消化性溃疡或胃炎组织中有7种存在端粒酶活性。在胃灌洗液中，在20例胃癌患者（80％），7例胃溃疡患者（28％）中检测到端粒酶，在正常受试者中未检测到端粒酶（P <0.001）。胃癌患者胃液端粒酶表达的敏感性，特异性，阳性预测值和阴性预测值分别为80％，84％，74％和88％。结论：与未胃癌患者相比，胃癌患者洗胃液中存在端粒酶活性，这可能是诊断胃癌的一种新方法。

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