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首页> 外文期刊>Anticancer Research: International Journal of Cancer Research and Treatment >The detection of microscopically disseminated cancer cells in the abdominal cavity by intraoperative lavage cytology combined with an immunocytochemical method in gastric cancer.
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The detection of microscopically disseminated cancer cells in the abdominal cavity by intraoperative lavage cytology combined with an immunocytochemical method in gastric cancer.

机译：胃癌术中灌洗细胞学结合免疫细胞化学方法检测腹腔镜下弥散性癌细胞。

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摘要

This study was conducted to clarify the possible role of the immunocytochemical examination of intraoperative lavage cytology in gastric cancer. The expression of CA19-9, STN, SLX and CEA in tissues were examined in 70 patients with advanced gastric cancer who underwent gastric resection. The tissue sections were processed with the hematoxylin and eosin staining and immunostaining using the avidin-biotin-peroxidase complex (ABC) method. Fifty one patients underwent the lavage cytology. The cytologic samples were stained by the conventional Papanicolau method and ABC immunocytochemical method. Expression of CEA was detected at obviously higher frequency than those of the 3 carbohydrate antigens. The method combined with 4 antibodies increased the detection rate to 97.2%. Conventional lavage cytology was positive in 16 out of 51 patients. The diagnosis of class III in four patients was changed to class V through the immunocytochemical examination. The immunocytochemical examination of lavage cytology is very useful to verify the microscopically disseminated cancer cells in gastric cancer.
机译：进行这项研究是为了阐明免疫细胞化学检查在胃癌术中灌洗细胞学检查中的可能作用。在70例行胃切除术的晚期胃癌患者中检测了CA19-9，STN，SLX和CEA在组织中的表达。用苏木精和曙红染色处理组织切片，并使用抗生物素蛋白-生物素-过氧化物酶复合物（ABC）方法进行免疫染色。 51名患者接受了灌洗细胞学检查。用常规的帕潘尼古劳法和ABC免疫细胞化学法对细胞学样品染色。 CEA的表达频率明显高于3种碳水化合物抗原的表达频率。结合4种抗体的方法将检测率提高到97.2％。 51例患者中有16例常规灌洗细胞学阳性。通过免疫细胞化学检查，四名患者的III级诊断改变为V级。灌洗细胞学的免疫细胞化学检查对于验证胃癌中显微镜下弥散的癌细胞非常有用。

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