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首页> 外文期刊>Журнал аналитической химии >Voltammetric determination of the components of biospecific deoxyribonucleic acid (DNA)-DNA antibody interaction as their Pt(II) complexes
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Voltammetric determination of the components of biospecific deoxyribonucleic acid (DNA)-DNA antibody interaction as their Pt(II) complexes

机译:伏安法测定生物特异性脱氧核糖核酸(DNA)-DNA抗体相互作用的成分为Pt(II)配合物

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摘要

A biochemical sensor based on a stationary mercury film electrode and a cellulose nitrate film containing either DNA molecules or molecules of antibodies to DNA was developed to determine the concentration of DNA (antibodies to DNA) and to diagnose autoimmune diseases. The peak currents of catalytic hydrogen liberation upon the formation of Pt(II) DNA (antibodies to DNA) complexes were used as the analytical signal. The detection limits were 0.075 and 10 #mu#g/mL for antibodies and DNA, respectively.
机译:开发了一种基于固定汞膜电极和硝酸纤维素膜的生化传感器,该膜包含DNA分子或抗DNA抗体分子,以确定DNA的浓度(DNA抗体)并诊断自身免疫性疾病。形成Pt(II)DNA(DNA抗体)复合物时催化氢释放的峰值电流用作分析信号。抗体和DNA的检出限分别为0.075和10#mu#g / mL。

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