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Lithostatine concentration is not useful for assessing the preanalytical variations in biobanked urine samples

机译:Statstatin浓度对评估生物库尿液样品的分析前变化没有帮助

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摘要

Proteomic research requires high-quality, standardized samples. Quality control (QC) biomarkers, which are sensitive to the collection, processing or storage conditions, would be useful tools to identify compromised samples. This study evaluates the usefulness of renal lithostatine as a QC tool for urine sample processing in daily biobank work. Four factors (pre-analytical variations) were examined for their effect on renal lithostatine as measured by ELISA: time from sample collection to centrifugation, number of specimen freeze-thaw cycles, specimen preservation with protease inhibitors, and the inclusion or exclusion of urinary sediment.
机译:蛋白质组学研究需要高质量的标准化样品。对收集,加工或储存条件敏感的质量控制(QC)生物标志物将是识别受损样品的有用工具。这项研究评估了肾利托他汀作为日常生物库工作中尿液样品处理的质量控制工具的有用性。通过ELISA测定了四个因素(分析前的变化)对肾利托他汀的影响:从样品收集到离心的时间,样品冻融循环的次数,样品中蛋白酶抑制剂的保存以及尿沉渣的包含或排除。

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