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首页> 外文期刊>Comparative Medicine >Estrogen-Responsive Transient Expression Assay Using a Brain Aromatase-Based Reporter Gene in Zebrafish (Danio rerio).
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Estrogen-Responsive Transient Expression Assay Using a Brain Aromatase-Based Reporter Gene in Zebrafish (Danio rerio).

机译：使用基于脑芳香酶的报道基因在斑马鱼中的雌激素响应性瞬时表达测定（斑马鱼）。

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Whereas endogenous estrogens play an important role in the development, maintenance, and function of female and male reproductive organs, xenoestrogens present in the environment disrupt normal endocrine function in humans and wildlife. Various in vivo and in vitro assays have been developed to screen these xenoestrogens. However, traditional in vivo assays are laborious and unsuitable for large-scale screening, and in vitro assays do not necessarily replicate in vivo functioning. To overcome these limitations, we developed a transient expression assay in zebrafish, into which a brain aromatase (cyp19a1b)-based estrogen-responsive reporter gene was introduced. In response to 17o-estradiol (10e M) and heptachlor (10e M), zebrafish embryos carrying the reporter construct expressed enhanced green fluorescent protein in the olfactory bulb, telencephalon, preoptic area, and mediobasal hypothalamus. This system will serve to model the in vivo conversion and breakdown of estrogenic compounds and thus provide a rapid preliminary screening method to estimate their estrogenicity.
机译：内源性雌激素在雌性和雄性生殖器官的发育，维持和功能中起着重要作用，而环境中存在的异种雌激素则破坏人类和野生生物的正常内分泌功能。已经开发了各种体内和体外测定法来筛选这些异雌激素。但是，传统的体内测定很费力并且不适合大规模筛选，并且体外测定不一定能复制体内功能。为了克服这些限制，我们开发了一种在斑马鱼中的瞬时表达测定法，其中引入了基于脑芳香化酶（cyp19a1b）的雌激素反应性报道基因。响应17o-雌二醇（10e M）和七氯（10e M），携带报告基因构建体的斑马鱼胚胎在嗅球，端脑，视前区和下丘脑下丘脑表达增强的绿色荧光蛋白。该系统将用于模拟体内雌激素化合物的转化和分解，从而提供一种快速的初步筛选方法以评估其雌激素性。

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