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首页> 外文期刊>Comparative Medicine >Experimental Infection of Mice with Hamster Parvovirus: Evidence for Interspecies Transmission of Mouse Parvovirus 3
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Experimental Infection of Mice with Hamster Parvovirus: Evidence for Interspecies Transmission of Mouse Parvovirus 3

机译：仓鼠细小病毒对小鼠的实验感染：小鼠细小病毒3种间传播的证据。

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Hamster parvovirus (HaPV) was isolated 2 decades ago from hamsters with clinical signs similar to those induced in hamsters experimentally infected with other rodent parvoviruses. Genetically, HaPV is most closely related to mouse parvovirus (MPV), which induces subclinical infection in mice. A novel MPV strain, MPV3, was detected recently in naturally infected mice, and genomic sequence analysis indicates that MPV3 is almost identical to HaPV. The goal of the present studies was to examine the infectivity of HaPV in mice. Neonatal and weanling mice of several mouse strains were inoculated with HaPV. Tissues, excretions, and sera were harvested at 1, 2, 4, and 8 wk after inoculation and evaluated by quantitative PCR and serologic assays specific for HaPV. Quantitative PCR detected viral DNA quantities that greatly exceeded the quantity of virus in inocula in multiple tissues of infected mice. Seroconversion to both nonstructural and structural viral proteins was detected in most immunocompetent mice 2 or more weeks after inoculation with HaPV. In neonatal SCID mice, viral transcripts were detected in lymphoid tissues by RT-PCR and viral DNA was detected in feces by quantitative PCR at 8 wk after inoculation. No clinical signs, gross, or histologic lesions were observed. These findings are similar to those observed in mice infected with MPV. These data support the hypothesis that HaPV and MPV3 are likely variants of the same viral species, for which the mouse is the natural rodent host with rare interspecies transmission to the hamster.
机译：仓鼠细小病毒（HaPV）于20年前从仓鼠中分离出来，其临床症状与实验性感染其他啮齿类细小病毒的仓鼠中诱导的症状相似。从基因上讲，HaPV与小鼠细小病毒（MPV）密切相关，后者可在小鼠中诱发亚临床感染。最近在自然感染的小鼠中检测到一种新型MPV株MPV3，并且基因组序列分析表明MPV3与HaPV几乎相同。本研究的目的是检查HaPV在小鼠中的感染性。 HaPV接种了几种小鼠品系的新生和断奶小鼠。接种后第1、2、4和8周收集组织，排泄物和血清，并通过定量PCR和HaPV特异的血清学分析进行评估。定量PCR检测到的病毒DNA量大大超过了被感染小鼠多个组织中接种物中的病毒量。接种HaPV后2周或更长时间，在大多数具有免疫能力的小鼠中都检测到了血清向非结构和结构病毒蛋白的血清转化。在新生SCID小鼠中，接种后8周通过RT-PCR在淋巴样组织中检测到病毒转录本，并通过定量PCR在粪便中检测到病毒DNA。没有观察到临床体征，肉眼或组织学病变。这些发现与感染MPV的小鼠相似。这些数据支持以下假设，即HaPV和MPV3可能是同一病毒物种的变体，为此，小鼠是天然啮齿动物宿主，极少种间传播给仓鼠。

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