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The Effect of Liposome Treatment on the Quality of Hypothermically Stored Red Blood Cells

机译：脂质体处理对低温保存的红细胞质量的影响

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Recent studies have demonstrated that liposome treatment of red blood cells (RBCs) leads to improved recovery and membrane integrity following cryopreservation protocols. However, the effect of liposome treatment on hypothermically stored RBCs has not been previously investigated. The current study has investigated whether liposome treatment could modify the membrane quality and deformability of hypothermically stored RBCs. Unilamellar liposomes were synthesized using an extrusion protocol. Three lipid bilayer compositions were investigated: 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC):PE:PS (8:1:1); 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC):PE:PS (8:1:1); and 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC):PE:PS (8:1:1). RBCs were treated with liposomes and subsequently stored for 42 days in HEPES-NaCl buffer and saline-adenine-glucose- mannitol. RBC quality was assessed by percent hemolysis, mean corpuscular volume (MCV), and RBC deformability (ektacytometry). DOPC and DMPC liposome treatment resulted in destabilization of the RBC membrane. Percent hemolysis values for DMPC-treated RBCs were higher than untreated controls throughout storage (P<0.05). DOPC-treated RBCs showed elevated levels of hemolysis compared to controls from day 21 of storage onward (P<0.05). In addition, DOPC and DMPC-treated RBCs were less deformable than untreated controls from days 21 (P = 0.02) and 14 (P<0.001) of storage onward respectively. [We suggest that these changes in RBC hemolysis and deformability are due to cholesterol extraction from the RBC membrane into the liposome fraction.] In contrast, DPPC-treated RBCs maintained hemolysis, MCV, and deformability values comparable to untreated controls. Future research addressing the optimal liposome composition for stabilizing the RBC membrane at cold temperatures could lead to effective strategies to combat the RBC membrane hypothermic storage lesion and ultimately improve the quality of hypothermically preserved blood.
机译：最近的研究表明，冷冻保存方案后，脂质体对红细胞（RBC）的处理可提高回收率和膜完整性。但是，脂质体处理对低温保存的红细胞的作用尚未进行过研究。目前的研究已经调查了脂质体处理是否可以改变低温保存的RBC的膜质量和可变形性。使用挤出方案合成单层脂质体。研究了三种脂质双层组合物：1,2-二棕榈酰-sn-甘油-3-磷酸胆碱（DPPC）：PE：PS（8：1：1）; 1,2-二油酰基-sn-甘油-3-磷酸胆碱（DOPC）：PE：PS（8：1：1）;和1,2-二肉豆蔻酰基-sn-甘油-3-磷酸胆碱（DMPC）：PE：PS（8：1：1）。用脂质体处理红细胞，然后在HEPES-NaCl缓冲液和盐水-腺嘌呤-葡萄糖-甘露醇中保存42天。 RBC的质量通过溶血百分比，平均红细胞体积（MCV）和RBC变形能力（流式细胞仪）进行评估。 DOPC和DMPC脂质体处理导致RBC膜不稳定。在整个存储过程中，DMPC处理的RBC的溶血百分比均高于未处理的对照组（P <0.05）。从储存开始第21天开始，与对照组相比，经DOPC处理的RBC表现出较高的溶血水平（P <0.05）。此外，分别从第21天（P = 0.02）和第14天（P <0.001）开始，DOPC和DMPC处理的RBC的变形性均低于未处理的对照。 （我们认为RBC溶血和可变形性的这些变化是由于胆固醇从RBC膜中提取到脂质体部分中所致。）相比之下，DPPC处理的RBC保持的溶血度，MCV和可变形性值与未处理的对照组相当。未来的研究针对在低温下稳定RBC膜的最佳脂质体组成，可能会导致有效的策略来对抗RBC膜的低温存储病变，并最终提高低温保存的血液的质量。

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