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Short-term stability study of RNA at room temperature

机译：室温下RNA的短期稳定性研究

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The quality of RNA preserved in different stabilization matrices was investigated after 2 weeks of storage at room temperature. RNA samples in RNAstable (Biomatrica), GenTegra (IntegenX), and RNAshell (Imagene) were compared to RNA stored at -80°C (the current gold standard for RNA preservation) and with liquid or dried RNA stored at room temperature without additives in this multi-center study. One center prepared all of the RNA samples, and five participating laboratories applied the samples to the matrices and stored them for 2 weeks at room temperature. Samples were shipped to three testing laboratories, where the 336 RNA samples were rehydrated and then analyzed for RNA recovery, purity, and integrity. Parallel RNA quality analyses and real-time PCR analyses were performed at each of the three testing laboratories. Each of the RNA matrices tested was shown to be fit-for-purpose for short-term room temperature storage in terms of total RNA recovery and rRNA integrity. All but one of the matrices was judged to be fit-for-purpose for mRNA integrity when assessed by real-time PCR analysis. In a follow-up study, RNase-contaminated samples were shown to provide accurate real-time PCR results when stored for up to 3.5 months in either RNAshell or RNAstable.
机译：在室温下保存2周后，研究了保存在不同稳定基质中的RNA的质量。将RNAstable（Biomatrica），GenTegra（IntegenX）和RNAshell（Imagene）中的RNA样品与-80°C（当前用于RNA保鲜的金标准）以及在室温下不添加添加剂的液态或干燥RNA一起存储的RNA进行比较这项多中心研究。一个中心准备了所有RNA样品，五个参与实验室将样品应用于基质，并在室温下保存2周。将样品运送到三个测试实验室，对336个RNA样品进行水合，然后分析其RNA回收率，纯度和完整性。在这三个测试实验室中的每一个都进行了平行RNA质量分析和实时PCR分析。就总RNA回收率和rRNA完整性而言，每种测试的RNA基质均适合短期室温存储。通过实时PCR分析评估时，除其中一种基质外，其他所有基质均被认为适合mRNA完整性。在后续研究中，被RNA酶污染的样品在RNAshell或RNAstable中保存长达3.5个月后，可提供准确的实时PCR结果。

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