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首页> 外文期刊>Biopreservation and biobanking >RNA Extracted From Blood Samples With a Rapid Automated Procedure Is Fit for Molecular Diagnosis or Minimal Residual Disease Monitoring in Patients With a Variety of Malignant Blood Disorders
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RNA Extracted From Blood Samples With a Rapid Automated Procedure Is Fit for Molecular Diagnosis or Minimal Residual Disease Monitoring in Patients With a Variety of Malignant Blood Disorders

机译：通过快速自动化程序从血样中提取的RNA适合于各种恶性血液病患者的分子诊断或最小残留疾病监测

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Scientific studies in oncology, cancer diagnosis, and monitoring tumor response to therapeutics currently rely on a growing number of clinico-pathological information. These often include molecular analyses. The quality of these analyses depends on both pre-analytical and analytical information and often includes the extraction of DNA and/or RNA from human tissues and cells. The quality and quantity of obtained nucleic acids are of utmost importance. The use of automated techniques presents several advantages over manual techniques, such as reducing technical time and thus cost, and facilitating standardization. The purpose of this study was to validate an automated technique for RNA extraction from cells of patients treated for various malignant blood diseases. A well-established manual technique was compared to an automated technique, in order to extract RNA from blood samples drawn for the molecular diagnosis of a variety of leukemic diseases or monitoring of minimal residual disease. The quality of the RNA was evaluated by real-time quantitative RT-PCR (RQ-PCR) analyses of the Abelson gene transcript. The results show that both techniques produce RNA with comparable quality and quantity, thus suggesting that an automated technique can be substituted for the reference and manual technique used in the daily routine of a molecular pathology laboratory involved in minimal residual disease monitoring. Increased costs of reagents and disposables used for automated techniques can be compensated by a decrease in human resource.
机译：肿瘤学，癌症诊断和监测肿瘤对治疗药物反应的科学研究目前依赖于越来越多的临床病理信息。这些通常包括分子分析。这些分析的质量取决于分析前和分析信息，并且通常包括从人体组织和细胞中提取DNA和/或RNA。获得的核酸的质量和数量至关重要。与手工技术相比，自动化技术的使用具有许多优势，例如减少了技术时间并因此降低了成本，并促进了标准化。这项研究的目的是验证一种从各种恶性血液病患者的细胞中提取RNA的自动化技术。为了从提取的血液样本中提取RNA进行各种白血病疾病的分子诊断或监测最小残留疾病，已将成熟的手动技术与自动化技术进行了比较。通过对Abelson基因转录本进行实时定量RT-PCR（RQ-PCR）分析来评估RNA的质量。结果表明，这两种技术都能产生质量和数量可比的RNA，从而表明可以用自动化技术代替分子病理学实验室日常工作中涉及的最小残留疾病监测的参考和手工技术。可以通过减少人力资源来弥补用于自动化技术的试剂和一次性用品的成本增加。

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