• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Biopreservation and biobanking >Bacterial contamination of blood DNA samples is associated with donor's health condition
【24h】

Bacterial contamination of blood DNA samples is associated with donor's health condition

机译：血液DNA样本的细菌污染与供体的健康状况有关

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Bacterial contamination often occurs in human blood DNA samples, possibly due to bacteremia or an inappropriate procedure during sample preparation. This study aimed at analyzing the clinical significance of bacterial DNA contamination in human blood DNA samples and to assess its influence on experimental data. DNA samples (N=1359) were randomly selected from population-based cohort samples to determine bacterial DNA contamination by polymerase chain reaction and direct DNA sequencing. Bacterial DNA contaminated samples (N=150) were then assessed for experimental quality of single nucleotide polymorphism (SNP) chip data, compared with uncontaminated DNA samples (N=1209). DNA sequencing data showed that a major source of bacterial contaminants was derived from Alcaligenes species. The occurrence of bacterial DNA contaminations was significantly associated with some clinical variables including a postprandial glucose level at 60min, % body fat, and waist-to-hip ratio. It was also found that there was no difference of SNP call rates between bacterial DNA contaminated samples and uncontaminated DNA samples. This study showed that bacterial DNA contamination in human blood samples was related to donor's health condition, suggesting that the occurrence of bacterial DNA contamination may provide useful health information of blood donors and a potential tool for human disease genomics.
机译：细菌污染经常在人血DNA样品中发生，可能是由于菌血症或样品制备过程中的不当操作所致。这项研究旨在分析人血DNA样品中细菌DNA污染的临床意义，并评估其对实验数据的影响。从基于人群的队列样本中随机选择DNA样本（N = 1359），以通过聚合酶链反应和直接DNA测序确定细菌DNA污染。然后评估细菌DNA污染样品（N = 150）与未污染DNA样品（N = 1209）相比的单核苷酸多态性（SNP）芯片数据的实验质量。 DNA测序数据表明，细菌污染物的主要来源是产碱菌物种。细菌DNA污染的发生与一些临床变量显着相关，包括60分钟餐后血糖水平，体脂％和腰臀比。还发现在细菌DNA污染的样品和未污染的DNA样品之间，SNP检出率没有差异。这项研究表明，人类血液样本中的细菌DNA污染与献血者的健康状况有关，这表明细菌DNA污染的发生可能为献血者提供有用的健康信息，并可能成为人类疾病基因组学的潜在工具。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号