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首页> 外文期刊>Biology of Reproduction: Offical Journal of the Society for the Study of Reproduction >A Dual Role for Melatonin in Medaka Ovulation: Ensuring Prostaglandin Synthesis and Actin Cytoskeleton Rearrangement in Follicular Cells
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A Dual Role for Melatonin in Medaka Ovulation: Ensuring Prostaglandin Synthesis and Actin Cytoskeleton Rearrangement in Follicular Cells

机译：褪黑素在Medaka排卵中的双重作用：确保卵泡细胞中前列腺素的合成和肌动蛋白的细胞骨架重排。

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摘要

Understanding the direct effects of melatonin on vertebrate ovulation remains a challenge. The present study provides the first characterization of the role of melatonin in ovulation using the teleost medaka. The melatonin receptor antagonist luzindole inhibited in vitro follicle ovulation. In the preovulatory follicles, arylalkylamine N-acetyltransferase 1a and hydroxyindole-O-methyltransferase 2, the enzymes responsible for melatonin synthesis, were expressed in the granulosa cells throughout the 24 h spawning cycle. The granulosa cells of the follicle also expressed the melatonin receptor 1a-a. An in vitro characterization study using medaka OLHNI-2 cells revealed that melatonin and luzindole act as an agonist and an antagonist, respectively, of the melatonin receptor. The intracellular cAMP levels in these cells were reduced after melatonin treatment. The expression of cytosolic phospholipase A2 group 4a (Pla2g4a), the enzyme producing arachidonic acid (cyclooxygenase-2 substrate), was inhibited in the granulosa cells in luzindole-treated follicles. Follicular prostaglandin E2 levels and in vitro follicle ovulation were suppressed in follicles isolated at 12 h prior to ovulation and incubated with the Pla2g4a inhibitor AACOCF3. The G-actin:F-actin ratios in follicular cells increased with approaching ovulation, but this increase was suppressed after luzindole treatment. The phosphorylation of moesin, an ezrin-radixin-moesin protein, was inhibited in the follicular cells in luzindole-treated follicles. These results indicate a dual role for melatonin in medaka ovuiation: melatonin ensures prostaglandin E2 synthesis throughout the spawning cycle and induces actin cytoskeleton rearrangement in the follicular cells at ovulation.
机译：了解褪黑激素对脊椎动物排卵的直接影响仍然是一个挑战。本研究首次提供了褪黑素在硬骨鱼中在排卵中的作用。褪黑激素受体拮抗剂卢辛多尔抑制体外卵泡排卵。在排卵前卵泡中，负责褪黑激素合成的芳基烷基胺N-乙酰基转移酶1a和羟基吲哚-O-甲基转移酶2在整个产卵周期中都表达于颗粒细胞中。卵泡的颗粒细胞也表达褪黑激素受体1a-a。使用medaka OLHNI-2细胞进行的体外表征研究表明，褪黑激素和luzindole分别充当褪黑激素受体的激动剂和拮抗剂。褪黑激素处理后，这些细胞中的细胞内cAMP水平降低。产花生四烯酸的酶（环氧合酶-2底物）胞质磷脂酶A2组4a（Pla2g4a）的表达在接受吲哚多酚处理的卵泡的颗粒细胞中受到抑制。排卵前12 h分离的卵泡中抑制了卵泡前列腺素E2水平和体外卵泡排卵，并与Pla2g4a抑制剂AACOCF3一起孵育。接近排卵时，卵泡细胞中的G-肌动蛋白：F-肌动蛋白比率增加，但在接受了吲哚多治疗后，这种增加被抑制了。在luzindole处理的卵泡的卵泡细胞中，moesin的磷酸化（一种ezrin-radixin-moesin蛋白）受到抑制。这些结果表明褪黑素在medaka排卵中具有双重作用：褪黑素可确保整个产卵周期中前列腺素E2的合成，并在排卵时诱导卵泡细胞中肌动蛋白细胞骨架重排。

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