High-throughput measurement of 25-hydroxyvitamin D by LC-MS/MS with separation of the C3-epimer interference for pediatric populations

Yang Yifei; Rogers Katherine; Wardle Robert; El-Khoury Joe M.

首页> 外文期刊>Clinica chimica acta: International journal of clinical chemistry and applied molecular biology >High-throughput measurement of 25-hydroxyvitamin D by LC-MS/MS with separation of the C3-epimer interference for pediatric populations

【24h】

High-throughput measurement of 25-hydroxyvitamin D by LC-MS/MS with separation of the C3-epimer interference for pediatric populations

机译：通过LC-MS / MS高通量测定25-羟基维生素D并分离C3表象素干扰儿科人群

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

Background: Liquid chromatography-tandem mass spectrometry (LC-MS/MS) is widely used for the measurement of 25-hydroxyvitamin D-3 (25OHD(3)) and 25-hydroxyvitamin D-2 (25OHD(2)) in blood. However, the presence of 25OHD(3) C3-epimer (3-epi-25OHD(3)) may cause interference and overestimation of the 25OHD(3) level. We developed a rapid and simple assay for measurement of 25OHD that separates this interference and to investigate its impact on 25OHD(3) measurement in adult and pediatric populations.

机译：背景：液相色谱-串联质谱法（LC-MS / MS）被广泛用于测量血液中的25-羟基维生素D-3（25OHD（3））和25-羟基维生素D-2（25OHD（2））。但是，存在25OHD（3）C3-epimer（3-epi-25OHD（3））可能会导致干扰和25OHD（3）水平的高估。我们开发了一种快速而简单的测定25OHD的方法，该方法可分离这种干扰并研究其对成人和儿科人群25OHD（3）测量的影响。

著录项

来源
《Clinica chimica acta: International journal of clinical chemistry and applied molecular biology》 |2016年第null期|共5页
作者
Yang Yifei; Rogers Katherine; Wardle Robert; El-Khoury Joe M.;
展开▼
作者单位

展开▼
收录信息
原文格式 PDF
正文语种 eng
中图分类诊断学;
关键词
Vitamin D; Liquid chromatography; Mass spectrometry; C3-epimer;

机译：维生素D;液相色谱;质谱;C3-差向异构体;

相似文献

外文文献
中文文献
专利

1. High-throughput measurement of 25-hydroxyvitamin D by LC-MS/MS with separation of the C3-epimer interference for pediatric populations [J] . Yang Yifei, Rogers Katherine, Wardle Robert, Clinica chimica acta: International journal of clinical chemistry and applied molecular biology . 2016,第Null期

机译：通过LC-MS / MS高通量测定25-羟基维生素D并分离C3表象素干扰儿科人群
2. Analytical measurement of serum 25-OH-vitamin D3, 25-OH-vitamin D2 and their C3-epimers by LC-MS/MS in infant and pediatric specimens [J] . YazdanpanahM., BaileyD., WalshW., Clinical Biochemistry . 2013,第13a14期

机译：LC-MS / MS法测定婴幼儿标本中血清25-OH-维生素D3、25-OH-维生素D2及其C3-受体的分析方法
3. Interference from 3-O-Methyldopa with Ultra-High Performance LC-MS/MS Measurements of Plasma Metanephrines: Chromatographic Separation Remains Important [J] . Peitzsch Mirko, Adaway Joanne E., Eisenhofer Graeme Clinical Chemistry: Journal of the American Association for Clinical Chemists . 2015,第7期

机译：3-O-甲基多巴对血浆间肾上腺素的超高性能LC-MS / MS测量的干扰：色谱分离仍然很重要
4. Approaches in Method Development and Determination of Endogenous 25-Hydroxyvitamin D3 in Rat Serum by Electrospray LC-MS/MS [C] . Rong Yi, Hong Zhang, Gina de Boer, American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics . 2010

机译：电气喷雾LC-MS / MS方法开发和测定大鼠血清内源25-羟基乙多素D3的方法
5. On-probe separations for MALDI-TOF-MS and LC-MS/MS of MHC peptides [D] . Brockman, Adam Hale 1998

机译：MHC肽的MALDI-TOF-MS和LC-MS / MS的探针分离
6. Determination of serum 25-hydroxyvitamin D status among population in southern China by a high accuracy LC-MS/MS method traced to reference measurement procedure [O] . Zhiliang Cai, Qiaoxuan Zhang, Ziqiang Xia, 2020

机译：高精度LC-MS / MS法测定中国南方人群血清25-羟维生素D含量
7. Harmonization study between LC-MS/MS and Diasorin RIA for measurement of 25-hydroxyvitamin D concentrations in a large population survey [O] . Berry Diane J., Dutton John, Fraser William D., 2017

机译：LC-MS / MS与Diasorin RIA之间的协调研究，用于在大规模人群调查中测量25-羟基维生素D的浓度

High-throughput measurement of 25-hydroxyvitamin D by LC-MS/MS with separation of the C3-epimer interference for pediatric populations

摘要

著录项

相似文献

相关主题

期刊订阅